Identification of lncRNA biomarkers in lung squamous cell carcinoma using comprehensive analysis of lncRNA mediated ceRNA network

被引：26
作者：
Li, Rui [1 ]
Yang, Yi-E [2 ]
Jin, Jia [3 ]
Zhang, Meng-Yu [1 ]
Liu, Xiao [1 ]
Liu, Xiao-Xia [1 ]
Yin, Yun-Hong [1 ]
Qu, Yi-Qing [1 ]
机构：
[1] Shandong Univ, Qilu Hosp, Dept Resp & Crit Care Med, Jinan 250012, Shandong, Peoples R China
[2] Qianfoshan Hosp Shandong Prov, Dept Clin Lab, Jinan, Shandong, Peoples R China
[3] Zhangqiu Dist Peoples Hosp Jinan, Dept Cardiol, Jinan, Shandong, Peoples R China
来源：
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY | 2019年 / 47卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Competing endogenous RNA; 6-lncRNA signature; lung squamous cell carcinoma; biomarker; EPITHELIAL-MESENCHYMAL TRANSITION; COMPETING ENDOGENOUS RNA; LONG NONCODING RNAS; TUMOR-SUPPRESSOR; DOWN-REGULATION; CANCER; METASTASIS; EXPRESSION; MIRNA; TCGA;
D O I：
10.1080/21691401.2019.1647225
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Long non-coding RNAs (lncRNAs) act as a member of competing endogenous RNAs (ceRNAs) and plays a significant role in tumorigenesis. The aim of this study was to identify potential lncRNA biomarkers for predicting the prognosis of lung squamous cell carcinoma (LUSC) using a comprehensive analysis of lncRNA mediated ceRNA network. Differentially expressed RNAs datasets were obtained using edge R package in 502 LUSC tissues and 49 adjacent non-LUSC tissues from the Cancer Genome Atlas (TCGA). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed to identify functional enrichment implication of lncRNA related differentially expressed mRNAs. Survival analysis was used Kaplan-Meier curve method. Univariate and multivariate Cox regression analysis were performed to construct a predictive model with lncRNA biomarkers. A total of 2185 lncRNAs, 170 miRNAs and 2053 mRNAs were differentially expressed between LUSC tissues and adjacent non-LUSC tissues. The novel constructed ceRNA network incorporated 184 LUSC-specific lncRNAs, 18 miRNAs, and 49 mRNAs. About 11 of 184 differentially expressed lncRNAs and 1 of 18 differentially expressed miRNAs and 5 of 49 differentially expressed mRNAs were conspicuously related to overall survival (p<.05). Univariate and multivariate cox regression analysis showed that 6 lncRNAs were retrieved to construct a predictive model to predict the overall survival in LUSC patients. In conclusion, CeRNAs contributed to the progression of LUSC and a model with 6 lncRNAs might be potential biomarker for predicting the prognosis of LUSC.
引用
收藏
页码：3246 / 3258
页数：13
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