Identification and quantitation of human amniotic fluid components using capillary zone electrophoresis

被引：8

作者：

Gao, Tao ^{[2
]}

Zablith, Nadine ^{[2
]}

Burns, David H. ^{[3
]}

Koski, Kristine G. ^{[2
]}

Skinner, Cameron D. ^{[1
]}

机构：

[1] Concordia Univ, Dept Chem & Biochem, Montreal, PQ H4B 1R6, Canada

[2] McGill Univ, Sch Dietet & Human Nutr, Montreal, PQ H9X 3V9, Canada

[3] McGill Univ, Dept Chem, Montreal, PQ H3A 2K6, Canada

来源：

ANALYTICAL BIOCHEMISTRY | 2009年 / 388卷 / 01期

关键词：

INFANT BIRTH-WEIGHT; PROTEOME; PREGNANCY; PROTEINS; PROFILE; FETAL;

D O I：

10.1016/j.ab.2009.02.011

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A capillary electrophoresis method was used to measure albumin, immunoglobulin G (IgG), transferrin, and uric acid in 230 amniotic fluid (AF) samples collected at 15.15 +/- 0.06 weeks gestation. Species were quantified by external calibration using thiamine as internal standard. All major components were detected within 10 min. Migration time reproducibility was 3.0% relative standard deviation (RSD) and normalized peak areas were 12% RSD or better at 190 nm from 81 measurements of a pooled AF sample. The separation profile was not affected by 10 h of storage at room temperature OF by 10 freeze-thaw cycles, suggesting that frozen AF samples are Suitable for protein biomarker Studies. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：155 / 157

页数：3

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