Automated, high-resolution cellular retention and uptake studies in vitro

被引:69
作者
Bjorke, Henrik [1 ]
Andersson, Karl [1 ]
机构
[1] Uppsala Univ, Div Biomed Radiat Sci, Uppsala, Sweden
关键词
cellular uptake; cellular retention; protein interactions; kinetics;
D O I
10.1016/j.apradiso.2006.03.002
中图分类号
O61 [无机化学];
学科分类号
070301 ; 081704 ;
摘要
This report describes an automated method for the measurements of cellular retention and uptake of radiolabeled proteins interacting with cell-surface receptors on intact cancer cells. A complete uptake and retention measurement was performed in one cell dish using a rotating radioimmunoassay (RIA) principle. Compared to common manual measurements, rotating RIA saved both labor time and reagents and provided real-time binding traces with superior time-resolution. The rotating RIA retention profiles for different interactions agreed with retention times reported in the literature. (c) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码:901 / 905
页数:5
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