Determination of Myricetin in Rat and Rabbit Plasma by UPLC-MS/MS and Application to Pharmacokinetic Study

被引：0

作者：

Cai, Xiao-jun ^{[1
]}

Yang, Qiu-jie ^{[1
]}

Chen, Da-hui ^{[1
]}

Hu, Shu-ping ^{[1
]}

Zheng, Jing ^{[1
]}

Shen, Jia-qi ^{[1
]}

Xiang, Zheng ^{[1
]}

机构：

[1] Wenzhou Med Univ, Sch Pharmaceut Sci, Wenzhou 325035, Peoples R China

来源：

LATIN AMERICAN JOURNAL OF PHARMACY | 2015年 / 34卷 / 07期

关键词：

Myricetin; UPLC-MS/MS; Pharmacokinetics; CANCER-CELLS; ANTIINFLAMMATORY ACTIVITY; FLAVONOID MYRICETIN; PICEATANNOL; QUERCETIN; APIGENIN; PATHWAY; LEAVES;

D O I：

暂无

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Myricetin is a member of the flavonoid class of polyphenolic compounds, with antioxidant properties. An ultra-performance liquid chromatography with tandem mass spectrometry (UPLC - MS/MS) method was established for the determination of myricetin in rat and-rabbit plasma. Myricetin was administered by sublingual intravenous and ear-vein in rats and rabbits, respectively. The plasma samples from the rats and rabbits were extracted by ethyl acetate. Chromatographic separation was achieved on a HSS T3 column and the analytes were detected by using electrospray ionization (ESI) source with a positive ion mode. The pharmacokinetic parameters were analyzed by drug and statistic software (DAS). The result showed the good linearity in the concentration range of 1-1000 ng/mL for rat and 5-1000 ng/mL for rabbit, respectively. The intra- and inter-day precision was within 7.8 % and the accuracy ranged from 85.0 % to 91.1 %. The simple, sensitive and rapid UPLC-MS/MS method was successfully applied to the pharmacokinetic study of myricetin in rat and rabbit.

引用

页码：1373 / 1379

页数：7

共 24 条

[1] Red wine consumption may affect sperm biology: The effects of different concentrations of the phytoestrogen Myricetin on human male gamete function [J].

Aquila, Saveria ;

Santoro, Marta ;

De Amicis, Francesca ;

Guido, Carmela ;

Bonofiglio, Daniela ;

Lanzino, Marilena ;

Cesario, Maria Grazia ;

Perrotta, Ida ;

Sisci, Diego ;

Morelli, Catia .

MOLECULAR REPRODUCTION AND DEVELOPMENT, 2013, 80 (02) :155-165

[2] The estrogenic effects of apigenin, phloretin and myricetin based on uterotrophic assay in immature Wistar albino rats [J].

Barlas, Nurhayat ;

Ozer, Saadet ;

Karabulut, Gozde .

TOXICOLOGY LETTERS, 2014, 226 (01) :35-42

[3] Effect of myricetin on deoxycorticosterone acetate (DOCA)-salt-hypertensive rats [J].

Borde, Pravin ;

Mohan, Mahalaxmi ;

Kasture, Sanjay .

NATURAL PRODUCT RESEARCH, 2011, 25 (16) :1549-1559

[4]

Chen R, 2014, MOL VIS, V20, P242

[5] Exploration of pro-oxidant and antioxidant activities of the flavonoid myricetin [J].

Chobot, Vladimir ;

Hadacek, Franz .

REDOX REPORT, 2011, 16 (06) :242-247

[6]

Choi H. N., 2013, FASEB J, V27, P8554

[7]

Dang Y, 2014, Drug Res (Stuttg), V64, P516, DOI 10.1055/s-0033-1363220

[8] Myricetin attenuates hyperinsulinemia-induced insulin resistance in skeletal muscle cells [J].

Ding, Ye ;

Dai, Xiao-qian ;

Zhang, Zhao-feng ;

Li, Yong .

EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2012, 234 (05) :873-881

[9] Effect of myricetin on blood pressure and metabolic alterations in fructose hypertensive rats [J].

Godse, Sandip ;

Mohan, Mahalaxmi ;

Kasture, Veena ;

Kasture, Sanjay .

PHARMACEUTICAL BIOLOGY, 2010, 48 (05) :494-498

[10] Myricetin suppresses lipoteichoic acid-induced interleukin-1β and cyclooxygenase-2 expression in human gingival fibroblasts [J].

Gutierrez-Venegas, Gloria ;

Alonso Luna, Oscar ;

Agustin Ventura-Arroyo, Jairo ;

Hernandez-Bermudez, Cristina .

MICROBIOLOGY AND IMMUNOLOGY, 2013, 57 (12) :849-856

← 1 2 3 →