Identification and distribution of Parkin in rat brain

被引：38

作者：

Horowitz, JM

Myers, J

Stachowiak, MK

Torres, G

机构：

[1] SUNY Buffalo, Dept Psychol, Behav Neurosci Program, Buffalo, NY 14214 USA

[2] SUNY Buffalo, Mol & Struct Neurobiol & Gene Therapy Program, Buffalo, NY 14214 USA

[3] SUNY Buffalo, Dept Anat & Cell Biol, Buffalo, NY 14214 USA

[4] Medaille Coll, Buffalo, NY 14214 USA

来源：

NEUROREPORT | 1999年 / 10卷 / 16期

关键词：

adrenals; cell degeneration; dopamine; immunoblotting; Parkinson's; serotonin;

D O I：

10.1097/00001756-199911080-00025

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

MUTATIONS within the amino acid sequence of Parkin, the encoded protein of the parkin gene, appear to trigger the degeneration of dopaminergic neurons in the substantia nigra. Here, the presence and anatomical distribution of Parkin within-the rat was examined. Immunoblot analysis of tissue homogenates showed two major bands at 50 and 44 kDa. Within the brain, Parkin-containing neurons were identified in the basal ganglia, including the substantia nigra and caudate-putamen. Parkin was visualized in the raphe nucleus, which as in the substantia nigra, was closely localized to monoaminergic-encoding neurons. In addition,Parkin was detected in laminar structures such as the cortex and hippocampus; a substantial number of Parkin-immunoreactive neurons was seen in the cerebellum as well, Parkin therefore is widely distributed in brain pathways implicated in the pathology of Parkinson's. disease. NeuroReport port 10:3393-3397 (C) 1999 Lippincott Williams & Wilkins.

引用

页码：3393 / 3397

页数：5

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