Trichinella spiralis muscle larvae release extracellular vesicles with immunomodulatory properties

被引：30

作者：

Kosanovic, Maja ^{[1
]}

Cvetkovic, Jelena ^{[1
]}

Gruden-Movsesijan, Alisa ^{[1
]}

Vasilev, Sasa ^{[1
]}

Svetlana, Milanovic ^{[2
]}

Ilic, Natasa ^{[1
]}

Sofronic-Milosavljevic, Ljiljana ^{[1
]}

机构：

[1] Univ Belgrade, INEP, Inst Applicat Nucl Energy, Banatska 31b, Belgrade 11080, Serbia

[2] Univ Belgrade, Fac Vet Med, Belgrade, Serbia

来源：

PARASITE IMMUNOLOGY | 2019年 / 41卷 / 10期

关键词：

ES L1; exosomes; Helminths; immunomodulation; muscle larvae; nematode; ANTIGENS; SURFACE;

D O I：

10.1111/pim.12665

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Aims Extracellular vesicles (EVs) represent a newly discovered but universal communication tool between cells or organisms. However, few data exist on nematode EVs and none for Trichinella spiralis. Here, we aimed to investigate whether T spiralis muscle larvae produce EVs, whether they carry immunomodulatory proteins and whether they have a role in immunomodulation as a component of excretory-secretory muscle larvae products (ES L1). Methods and results EVs were enriched from conditioned medium of T spiralis muscle larvae. Transmission electron microscopy images showed T spiralis EVs to be 30-80 nm in size, and Western blot confirmed the presence of two out of three glycoproteins with the immunodominant epitope characteristic for muscle larvae of the genus Trichinella. Using a peripheral blood mononuclear cell (PBMC) stimulation assay, it was shown that these EVs elevated production of IL10 and IL6. Conclusion T spiralis muscle larvae produce EVs. Those EVs carry immunomodulatory proteins and have the capacity independently to induce regulatory responses in the same way as the T spiralis excretory-secretory muscle larvae products from which they were isolated.

引用

页数：5

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