In Vitro Cytotoxicity Study of Green Synthesized Copper Nanoparticles

被引：0

作者：

Shobha, G. ^{[1
]}

Sagar, S. ^{[1
]}

Shashidhara, K. S. ^{[2
]}

Mahadimane, Vishwaprakash ^{[3
]}

Ananda, S. ^{[1
]}

机构：

[1] Sapthagiri Coll Engn, Dept Biotechnol, Bangalore 560057, Karnataka, India

[2] Univ Agr Sci Bengaluru, Coll Agr Hassan, Dept Genet & Plant Breeding, Bengaluru 573225, India

[3] Alvas Ctr Post Grad Studies & Res, Dept Appl Zool, Moodbidri, Dakshina Kannan, India

来源：

RESEARCH JOURNAL OF BIOTECHNOLOGY | 2019年 / 14卷 / 08期

关键词：

Copper nanoparticle; Comet assay; DNA fragmentation; MTT assay; Tumour cells; LUNG-CANCER; TOXICITY;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In our earlier study, we reported the green synthesis of copper nanoparticles with antimicrobial activity. Here we report the cytotoxicity and genotoxicity potency of the green synthesized copper nanoparticles on cancer cells MCF-7. The different cell lines experimental results in our study have shown that the cells morphology changed significantly for cancer lines MCF-7 upon treatment with copper nanoparticles and IC50 dose for MCF-7 cells was found to be 1.71 mu g ml(-1) by MTT assay. At this concentration, copper nanoparticles had shown no cytotoxicity effect on normal cell line (3T3L1). The dual staining (Acridine orange/Ethidium Bromide) showed various degrees of accumulation of nanoparticles by apoptotic tumour cells with increasing concentration of copper nanoparticles. The comet assay and DNA fragmentation assay by gel electrophoresis on cancer cell line MCF-7 showed the chromosomal condensation and fragmentation of DNA after treatment with the copper nanoparticles.

引用

页码：105 / 111

页数：7

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