Novel SCRG1/BST1 axis regulates self-renewal, migration, and osteogenic differentiation potential in mesenchymal stem cells

被引：55

作者：

Aomatsu, Emiko ^{[1
,2
]}

Takahashi, Noriko ^{[1
]}

Sawada, Shunsuke ^{[3
,4
]}

Okubo, Naoto ^{[1
]}

Hasegawa, Tomokazu ^{[5
]}

Taira, Masayuki ^{[6
]}

Miura, Hiroyuki ^{[2
]}

Ishisaki, Akira ^{[1
]}

Chosa, Naoyuki ^{[1
]}

机构：

[1] Iwate Med Univ, Dept Biochem, Div Cellular Biosignal Sci, Yahaba, Iwate 0283694, Japan

[2] Iwate Med Univ, Sch Dent, Dept Dev Oral Hlth Sci, Div Orthodont, Morioka, Iwate 0208505, Japan

[3] Iwate Med Univ, Sch Dent, Dept Conservat Dent, Div Periodontol, Morioka, Iwate 0208505, Japan

[4] Iwate Med Univ, Sch Dent, Clin Res Lab, Morioka, Iwate 0208505, Japan

[5] Tokushima Univ Hosp, Dept Pediat Dent, Tokushima 7708504, Japan

[6] Iwate Med Univ, Dept Biomed Engn, Yahaba, Iwate 0283694, Japan

来源：

SCIENTIFIC REPORTS | 2014年 / 4卷

关键词：

HUMAN BONE-MARROW; FOCAL ADHESION KINASE; STROMAL CELLS; IN-VITRO; CD157; GENE; EXPRESSION; SCRAPIE; BST-1; REGENERATION;

D O I：

10.1038/srep03652

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Human mesenchymal stem cells (hMSCs) remodel or regenerate various tissues through several mechanisms. Here, we identified the hMSC-secreted protein SCRG1 and its receptor BST1 as a positive regulator of self-renewal, migration, and osteogenic differentiation. SCRG1 and BST1 gene expression decreased during osteogenic differentiation of hMSCs. Intriguingly, SCRG1 maintained stem cell marker expression (Oct-4 and CD271/LNGFR) and the potentials of self-renewal, migration, and osteogenic differentiation, even at high passage numbers. Thus, the novel SCRG1/BST1 axis determines the fate of hMSCs by regulating their kinetic and differentiation potentials. Our findings provide a new perspective on methods for ex vivo expansion of hMSCs that maintain native stem cell potentials for bone-forming cell therapy.

引用

页数：9

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