Development of luminescent nanoswitch for sensing of alkaline phosphatase in human serum based onAl3+-PPi interaction and Cu NCs with AIE properties

被引：37

作者：

Geng, Fenghua ^{[1
]}

Zou, Changpeng ^{[2
]}

Liu, Jinhua ^{[2
]}

Zhang, Qianchen ^{[2
]}

Guo, Xueying ^{[2
]}

Fan, Yunchang ^{[3
]}

Yu, Haidong ^{[2
]}

Yang, Sheng ^{[4
]}

Liu, Zhipeng ^{[2
]}

Li, Lin ^{[2
]}

机构：

[1] Shangqiu Normal Univ, Henan Key Lab Chemo Biosensing & Chemometr, Henan Joint Int Res Lab Chemo Biosensing & Early, Shangqiu 476000, Peoples R China

[2] Nanjing Tech Univ, NanjingTech, KLOFE, IAM,Jiangsu Natl Synerget Innovat Ctr Adv Mat SIC, 30 South Puzhu Rd, Nanjing 211816, Jiangsu, Peoples R China

[3] Henan Polytech Univ, Coll Chem & Chem Engn, Jiaozuo 454003, Henan, Peoples R China

[4] Changsha Univ Sci & Technol, Sch Chem & Food Engn, Changsha 410114, Hunan, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2019年 / 1076卷

基金：

国家重点研发计划; 美国国家科学基金会;

关键词：

Cu nanoclusters; Luminescent nanoswitch; ALP; Aggregation induced emission (AIE); AGGREGATION-INDUCED EMISSION; TIME FLUORESCENCE ASSAY; IN-SITU GROWTH; COPPER NANOCLUSTERS; SENSITIVE DETECTION; GOLD NANORODS; QUANTUM DOTS; NANOPARTICLES; PYROPHOSPHATE; PROBE;

D O I：

10.1016/j.aca.2019.05.026

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

As an important biomarker, alkaline phosphatase (ALP) is one of the most commonly assayed enzymes in clinical practice. Here a novel turn-on fluorescent nanoswitch for ALP assay was suggested. The nanoswitch was easily constructed via two high-affinity ligands between GSH and Al3+ and PPi and Al3+ based on the difference in the affinity. The primary fluorescence of as-prepared GSH capped Cu nanoclusters (NCs) turned on first upon the Al3+ addition due to the Al3+ induced aggregation induced emission (AIE) enhancement based on the high affinity of GSH and Al3+. The presence of PPi then made Al3+ desorb from the surface of GSH capped Cu NCs due to the higher affinity of PPi and Al3+. As a result, the fluorescence of the Cu NCs was quenched. ALP could hydrolyze PPi into phosphate, destroying the PPi-Al3+ complex and releasing Al3+. Thus, the Al3+ binds to GSH again and the fluorescence was restored. The nanoswitch was demonstrated to be sensitive and selective for ALP assay and was successfully used for the ALP assay in the human serum. (C) 2019 Elsevier B.V. All rights reserved.

引用

页码：131 / 137

页数：7

共 55 条

[1] Synthesis and Functionalization of Stable and Bright Copper Nanoclusters by In Situ Generation of Silica Shells for Bioimaging and Biosensing [J].