Hydrothermal fabrication of magnetic mesoporous carbonated hydroxyapatite microspheres: biocompatibility, osteoinductivity, drug delivery property and bactericidal property

被引:45
作者
Guo, Ya-Ping [1 ,2 ]
Long, Teng [3 ]
Tang, Sha [1 ,2 ]
Guo, Ya-Jun [1 ,2 ]
Zhu, Zhen-An [3 ]
机构
[1] Shanghai Normal Univ, Educ Minist, Key Lab Resource Chem, Shanghai 200234, Peoples R China
[2] Shanghai Normal Univ, Shanghai Key Lab Rare Earth Funct Mat, Shanghai 200234, Peoples R China
[3] Shanghai Jiao Tong Univ, Sch Med, Shanghai Peoples Hosp 9, Dept Orthoped Surg,Shanghai Key Lab Orthoped Impl, Shanghai 200011, Peoples R China
关键词
PULSED ELECTROMAGNETIC-FIELDS; BONE MORPHOGENETIC PROTEIN-2; IN-VITRO CHARACTERIZATION; CONTROLLED-RELEASE; OSTEOPROGENITOR RESPONSE; HYDROXYCARBONATE APATITE; BIOFILM FORMATION; GENE-EXPRESSION; CARRIER SYSTEM; CELL-ADHESION;
D O I
10.1039/c3tb21829e
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
0805 ; 080501 ; 080502 ;
摘要
Implant-associated infection is a serious problem in orthopaedic surgery. Ideal bone filling materials should not only possess excellent biocompatibility, but also have good anti-infection property and osteoinductivity. Herein, magnetic mesoporous carbonated hydroxyapatite microspheres (MHMs) have been fabricated according to the following stages: (i) preparation of CaCO3/Fe3O4 microspheres; and (ii) hydrothermal transformation of magnetic calcium carbonate microspheres into MHMs. MHMs possess well-defined 3D nanostructures constructed by nanoplates as building blocks. The mesopores and macropores exist in and among the nanoplates, respectively. The porous structure makes the MHMs possess a great drug loading efficiency of 73-82%. Gentamicin-loaded MHMs display a sustained drug release property, and the controlled release of gentamicin can prevent biofilm formation against S. epidermidis. Moreover, the MHMs possess a magnetic property with a saturation magnetization strength of 3.98 emu g(-1) because Fe3O4 nanoparticles are dispersed in the microspheres. The in vitro cell tests indicate that the magnetic nanoparticles in the MHMs not only promote the cell adhesion and proliferation of human bone marrow stromal cells (hBMSCs), but also stimulate the osteogenic differentiation. MHMs exhibit excellent biocompatibility, osteoinductivity, drug delivery property and bactericidal property, so they have great application potential for the treatment of complicated bone defects.
引用
收藏
页码:2899 / 2909
页数:11
相关论文
共 90 条
[1]   SPECTRA STRUCTURE CORRELATIONS IN HYDROXY AND FLUORAPATITE [J].
BADDIEL, CB ;
BERRY, EE .
SPECTROCHIMICA ACTA, 1966, 22 (08) :1407-&
[2]   Silica particles:: A novel drug-delivery system [J].
Barbé, C ;
Bartlett, J ;
Kong, LG ;
Finnie, K ;
Lin, HQ ;
Larkin, M ;
Calleja, S ;
Bush, A ;
Calleja, G .
ADVANCED MATERIALS, 2004, 16 (21) :1959-1966
[3]  
BASSETT CAL, 1989, CLIN ORTHOP RELAT R, P172
[4]   Morphology of sol-gel derived nano-coated coralline hydroxyapatite [J].
Ben-Nissan, B ;
Milev, A ;
Vago, R .
BIOMATERIALS, 2004, 25 (20) :4971-4975
[5]   A novel route in bone tissue engineering: Magnetic biomimetic scaffolds [J].
Bock, N. ;
Riminucci, A. ;
Dionigi, C. ;
Russo, A. ;
Tampieri, A. ;
Landi, E. ;
Goranov, V. A. ;
Marcacci, M. ;
Dediu, V. .
ACTA BIOMATERIALIA, 2010, 6 (03) :786-796
[6]   Calcium phosphate ceramic systems in growth factor and drug delivery for bone tissue engineering: A review [J].
Bose, Susmita ;
Tarafder, Solaiman .
ACTA BIOMATERIALIA, 2012, 8 (04) :1401-1421
[7]   The influence of crystallised Fe3O4 on the magnetic properties of coprecipitation-derived ferrimagnetic glass-ceramics [J].
Bretcanu, O ;
Spriano, S ;
Verné, E ;
Cöisson, M ;
Tiberto, P ;
Allia, P .
ACTA BIOMATERIALIA, 2005, 1 (04) :421-429
[8]  
Cabanillas PF, 2000, INT J PHARM, V209, P15
[9]   Exposed hydroxyapatite particles on the surface of photo-crosslinked nanocomposites for promoting MC3T3 cell proliferation and differentiation [J].
Cai, Lei ;
Guinn, Angela S. ;
Wang, Shanfeng .
ACTA BIOMATERIALIA, 2011, 7 (05) :2185-2199
[10]   Antibiotic-loaded biomaterials and the risks for the spread of antibiotic resistance following their prophylactic and therapeutic clinical use [J].
Campoccia, Davide ;
Montanaro, Lucio ;
Speziale, Pietro ;
Arciola, Carla Renata .
BIOMATERIALS, 2010, 31 (25) :6363-6377