Stern-Volmer quenching and binding constants of 10-alkyl-9(10H)-acridone probes in SDS and BSA

被引:38
作者
Lunardi, CN [1 ]
Bonilha, JBS [1 ]
Tedesco, AC [1 ]
机构
[1] Univ Sao Paulo, FFCLRP, Dept Chem, BR-14040901 Ribeirao Preto, SP, Brazil
基金
巴西圣保罗研究基金会;
关键词
fluorescence; micelle; bovine serum albumin; 10-alkyl-9(10H)-acridone;
D O I
10.1016/S0022-2313(02)00227-2
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
Lucigenin (bis-N-methylacridinium nitrate) is an acridine-based probe that has been extensively used to detect reactive oxygen species (ROs) and has been shown to generate chemiluminescence in many biological materials. N-methylacridone is known to be the main emitter of the lucigenin's chemiluminescent system. This work investigates the distribution of synthesized 10-alkyl-9(10H)-acridone (-methyl, -butyl and -decyl) probes and quenchers (Cu2+ MV2+, BeV2+, Propy(+) and DoPy(+) in anionic micellar solution of sodium dodecyl sulfate (SDS). The binding ability of 10-alkyl-9(]OH)-acridone to a serum lipoprotein (BSA) was investigated. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:61 / 71
页数:11
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