Concordance study between one-step nucleic acid amplification and morphologic techniques to detect lymph node metastasis in papillary carcinoma of the thyroid

被引：21

作者：

del Carmen, Sofia ^{[1
]}

Gatius, Sonia ^{[2
]}

Franch-Arcas, Guzman ^{[1
]}

Antonio Baena, Jose ^{[2
]}

Gonzalez, Oscar ^{[3
]}

Zafon, Carlos ^{[3
]}

Cuevas, Dolors ^{[2
]}

Valls, Joan ^{[2
]}

Perez, Angustias ^{[1
]}

Martinez, Mercedes ^{[1
]}

Ros, Susana ^{[2
]}

Garcia Macias, Carmen ^{[1
]}

Iglesias, Carmela ^{[3
]}

Matias-Guiu, Xavier ^{[2
]}

de Alava, Enrique ^{[1
]}

机构：

[1] Hosp Univ Salamanca, Salamanca 37007, Spain

[2] Hosp Univ Arnau de Vilanova Lleida, IRB Lleida, Lleida 25198, Spain

[3] Hosp Valle De Hebron, VHIR, Barcelona 08035, Spain

来源：

HUMAN PATHOLOGY | 2016年 / 48卷

关键词：

Papillary carcinoma; Thyroid; Lymph node; OSNA; Metastasis; BREAST-CANCER PATIENTS; NECK DISSECTION; OSNA; RECURRENCE; MORBIDITY; DIAGNOSIS; CK19;

D O I：

10.1016/j.humpath.2015.09.020

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Tumor resection in papillary thyroid carcinoma (PTC) is often accompanied by lymph node (LN) removal of the central and lateral cervical compartments. One-step nucleic acid amplification (OSNA) is a polymerase chain reaction based technique that quantifies cytokeratin 19 (CK19) messenger RNA copies. Our aim is to assess the value of OSNA in detection of LN metastases in PTC, in comparison with imprints and microscopic analysis of formalin-fixed, paraffin-embedded (FFPE) tissue. A total of 387 LNs from 37 patients were studied. From each half LN, 2 imprints were taken and analyzed with hematoxylin and eosin (H&E) and CK19 immunostaining. One half of the LN was submitted to OSNA and one half to FFPE processing and H&E and CK19 staining. For concordance analysis, every single LN was considered as a case. A group of 11 cases with discordant results between OSNA and H&E/CK19 FFPE sections were subjected to additional FFPE serial sectioning and H&E and CK19 staining. We found a high degree of concordance between the assays used, with sensitivities ranging from 0.81 to 0.95, and specificities ranging from 0.87 and 0.98. OSNA allowed upstaging of patients from pN0 to pN1, in comparison with standard pathologic analysis. Identification of a metastatic LN with more than 15000 CK19 messenger RNA copies predicted the presence of a second LN with macrometastasis (<5000 copies). In summary, the study shows that OSNA application in sentinel or suspicious LN may be helpful in assessing nodal status in PTC patients. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：132 / 141

页数：10

共 23 条

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