Polymorphic insertions and deletions in parabasalian enolase genes

被引:13
作者
Keeling, PJ [1 ]
机构
[1] Univ British Columbia, Dept Bot, Canadian Inst Adv Res, Vancouver, BC V6T 1Z4, Canada
关键词
parabasalia; enolase; insertion; deletion; phylogeny;
D O I
10.1007/s00239-003-2577-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Insertions and deletions in gene sequences have been used as characters to infer phylogenetic relationships and, like any character, the information they contain varies in utility between different levels of evolution. In one case, the absence of two otherwise highly conserved deletions in the enolase genes of parabasalian protists has been interpreted as a primitive characteristic that suggests these were among the first eukaryotes. Here, semi-environmental 3'-RACE was used to sample enolases from parabasalia in the hindgut of the termite Zootermopsis angusticolis to examine the conservation of this character within the parabasalia. Parabasalian homologues were found to be polymorphic for these deletions, and the phylogeny of parabasalian enolases shows that the deletion-possessing genes branch within deletion-lacking genes (i.e., they did not form two clearly distinct groups). Phylogenetic incongruence was detected in the carboxy-terminal third of the sequence (in the region of the deletions), but there is no unambiguous evidence for recombination. The polymorphism of this character discredits these deletions as strong evidence for the early origin of parabasalia, although the complex distribution makes it impossible to state whether parabasalian enolases were ancestrally like those of other eukaryotes. These observations stress the importance of strong corroborating evidence when considering insertion and deletion data, and raises some interesting questions about the apparent variation in degree of conservation of these deletions between different eukaryotic groups.
引用
收藏
页码:550 / 556
页数:7
相关论文
共 50 条
[41]   A comprehensive study of small non-frameshift insertions/deletions in proteins and prediction of their phenotypic effects by a machine learning method (KD4i) [J].
Bermejo-Das-Neves, Carlos ;
Nguyen, Hoan-Ngoc ;
Poch, Olivier ;
Thompson, Julie D. .
BMC BIOINFORMATICS, 2014, 15
[42]   cDNA cloning and expression pattern of two enolase genes from the Chinese oak silkworm, Antheraea pernyi [J].
Liu, Yanqun ;
Li, Yuping ;
Wang, Huan ;
Xia, Runxi ;
Li, Xisheng ;
Wan, Haolei ;
Qin, Li ;
Jiang, Defu ;
Lu, Cheng ;
Xiang, Zhonghuai .
ACTA BIOCHIMICA ET BIOPHYSICA SINICA, 2010, 42 (11) :816-826
[43]   Bayesian EM Algorithm for Scoring Polymorphic Deletions From SNP Data and Application to a Common CNV on 8q24 [J].
Zoellner, Sebastian ;
Su, Gang ;
Stewart, William C. L. ;
Chen, Yi ;
McInnis, Melvin G. ;
Burmeister, Margit .
GENETIC EPIDEMIOLOGY, 2009, 33 (04) :357-368
[44]   Deletions and Duplications of Developmental Pathway Genes in 5q31 Contribute to Abnormal Phenotypes [J].
Rosenfeld, Jill A. ;
Drautz, Joanne Milisa ;
Clericuzio, Carol L. ;
Cushing, Tom ;
Raskin, Salmo ;
Martin, Judith ;
Tervo, Raymond C. ;
Pitarque, Jose A. ;
Nowak, Dorota M. ;
Karolak, Justyna A. ;
Lamb, Allen N. ;
Schultz, Roger A. ;
Ballif, Blake C. ;
Bejjani, Bassem A. ;
Gajecka, Marzena ;
Shaffer, Lisa G. .
AMERICAN JOURNAL OF MEDICAL GENETICS PART A, 2011, 155A (08) :1906-1916
[45]   New recurrent deletions in the PPARγ and TP53 genes are associated with childhood myelodysplastic syndrome [J].
Silveira, Cassia G. T. ;
Oliveira, Fabio M. ;
Valera, Elvis T. ;
Ikoma, Maura R. V. ;
Borgonovo, Tamara ;
Cavalli, Iglenir J. ;
Tone, Luiz G. ;
Rogatto, Silvia R. .
LEUKEMIA RESEARCH, 2009, 33 (01) :19-27
[46]   Directed formation of chromosomal deletions in Salmonella typhimurium:: targeting of specific genes induced during infection [J].
Julio, SM ;
Conner, CP ;
Heithoff, DM ;
Mahan, MJ .
MOLECULAR AND GENERAL GENETICS, 1998, 258 (1-2) :178-181
[47]   Characterization of multiple enolase genes from Trichomonas vaginalis. Potential novel targets for drug and vaccine design [J].
Mirasol-Melendez, Elibeth ;
Brieba, Luis G. ;
Diaz-Quezada, Corina ;
Lopez-Hidalgo, Marisol ;
Figueroa-Angulo, Elisa E. ;
Avila-Gonzalez, Leticia ;
Arroyo-Verastegui, Rossana ;
Benitez-Cardoza, Claudia G. .
PARASITOLOGY INTERNATIONAL, 2018, 67 (04) :444-453
[48]   Characteristics of BCR-ABL kinase domain mutations in chronic myeloid leukemia from India: not just missense mutations but insertions and deletions are also associated with TKI resistance [J].
Patkar, Nikhil ;
Ghodke, Kiran ;
Joshi, Swapnali ;
Chaudhary, Shruti ;
Mascerhenas, Russel ;
Dusseja, Sona ;
Mahadik, Shashikant ;
Gaware, Sheetal ;
Tembhare, Prashant ;
Gujral, Sumeet ;
Kabre, Sharayu ;
Kadam-Amare, Pratibha ;
Jain, Hasmukh ;
Dangi, Uma ;
Bagal, Bhausaheb ;
Khattry, Navin ;
Sengar, Manju ;
Arora, Brijesh ;
Narula, Gaurav ;
Banavali, Shripad ;
Menon, Had ;
Subramanian, Papagudi Ganesan .
LEUKEMIA & LYMPHOMA, 2016, 57 (11) :2653-2660
[49]   Multiple independent insertions of 5S rRNA genes in the spliced-leader gene family of trypanosome species [J].
Beauparlant, Marc A. ;
Drouin, Guy .
CURRENT GENETICS, 2014, 60 (01) :17-24
[50]   Genotyping by PCR-ELISA of a complex polymorphic region that contains one to four copies of six highly homologous human VH3 genes [J].
Cho, CS ;
Wang, XF ;
Zhao, Y ;
Carson, DA ;
Chen, PP .
PROCEEDINGS OF THE ASSOCIATION OF AMERICAN PHYSICIANS, 1997, 109 (06) :558-564