Fingerprinting of Psychoactive Drugs in Zebrafish Anxiety-Like Behaviors

被引：80

作者：

Maximino, Caio ^{[1
]}

Batista da Silva, Annanda Waneza ^{[2
]}

Araujo, Juliana ^{[2
]}

Lima, Monica Gomes ^{[1
]}

Miranda, Vanessa ^{[2
]}

Puty, Bruna ^{[3
]}

Benzecry, Rances ^{[2
]}

Wanderley Picanco-Diniz, Domingos Luiz ^{[4
]}

Gouveia, Amauri, Jr. ^{[5
]}

Matos Oliveira, Karen Renata ^{[3
]}

Herculano, Anderson Manoel ^{[3
]}

机构：

[1] Univ Estado Para, Ctr Ciencias Biol & Saude, Dept Morfol & Ciencias Fisiol, Lab Neurociencias & Comportamento Frederico Graef, Maraba, Para, Brazil

[2] Fed Univ Para, Inst Ciencias Biol, BR-66059 Belem, Para, Brazil

[3] Fed Univ Para, Inst Ciencias Biol, Lab Neuroendocrinol, BR-66059 Belem, Para, Brazil

[4] Univ Fed Oeste Para, Oriximina, Para, Brazil

[5] Fed Univ Para, Lab Neurociencias & Comportamento, Nucleo Pesquisa & Teoria Comportamento, BR-66059 Belem, Para, Brazil

来源：

PLOS ONE | 2014年 / 9卷 / 07期

关键词：

DANIO-RERIO; ANIMAL-MODELS; ADULT ZEBRAFISH; DISCOVERY; SCOTOTAXIS; IDENTIFICATION; MODULATION; LIGHT/DARK; VERTEBRATE; PREFERENCE;

D O I：

10.1371/journal.pone.0103943

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A major hindrance for the development of psychiatric drugs is the prediction of how treatments can alter complex behaviors in assays which have good throughput and physiological complexity. Here we report the development of a medium-throughput screen for drugs which alter anxiety-like behavior in adult zebrafish. The observed phenotypes were clustered according to shared behavioral effects. This barcoding procedure revealed conserved functions of anxiolytic, anxiogenic and psychomotor stimulating drugs and predicted effects of poorly characterized compounds on anxiety. Moreover, anxiolytic drugs all decreased, while anxiogenic drugs increased, serotonin turnover. These results underscore the power of behavioral profiling in adult zebrafish as an approach which combines throughput and physiological complexity in the pharmacological dissection of complex behaviors.

引用

页数：8

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