Mapping toll-like receptor signaling pathway genes of Zhikong scallop (Chlamys farreri) with FISH

被引：8

作者：

Zhao Bosong ^{[1
]}

Zhao Liang ^{[1
]}

Liao Huan ^{[1
]}

Cheng Jie ^{[1
]}

Lian Shanshan ^{[1
]}

Li Xuan ^{[1
]}

Huang Xiaoting ^{[1
]}

Bao Zhenmin ^{[1
]}

机构：

[1] Ocean Univ China, Coll Marine Life Sci, Minist Educ, Key Lab Marine Genet & Breeding, Qingdao 266003, Peoples R China

来源：

JOURNAL OF OCEAN UNIVERSITY OF CHINA | 2015年 / 14卷 / 06期

基金：

中国国家自然科学基金;

关键词：

immunogenetics; Chlamys farreri; TLR signaling pathway; FISH; MESSENGER-RNA EXPRESSION; MOLECULAR-CLONING; INNATE IMMUNITY; CDNA CLONING; IDENTIFICATION; HOMOLOG; PROTEIN;

D O I：

10.1007/s11802-015-2643-8

中图分类号：

P7 [海洋学];

学科分类号：

0707 ;

摘要：

Toll-like receptor (TLR) signaling pathway plays a pivotal role in the innate immune system. Studies on TLR signaling pathway genes in Zhikong scallop (Chlamys farreri) have mainly focused on sequence analysis and expression profiling, no research has been carried out on their localization. The chromosomal position of TLR signaling pathway genes can be valuable for assemblying scallop genome and analysizing gene regulatory networks. In the present study, five key TLR signaling pathway genes (CfTLR, CfMyd88, CfTRAF6, CfNF kappa B, and CfI kappa B) containing bacterial artificial chromosomes (BACs) were isolated and physically mapped through fluorescence in situ hybridization on five non-homologous chromosome pairs, showing a similar distribution to another five model species. The isolation and mapping of these key immune genes of C. farreri will aid to the research on innate immunity, assignment of interested genes to chromosomes, and integration of physical, linkage and cytogenetic maps of this species.

引用

页码：1075 / 1081

页数：7

共 36 条

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