The MALDI TOF mass spectrometry:: Rapid qualitative determination of expression of recombinant proteins in Escherichia coli

被引：0

作者：

Serebryakova, MV ^{[1
]}

Rogov, SP ^{[1
]}

Shevalier, AF ^{[1
]}

Grachev, SA ^{[1
]}

机构：

[1] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117871, Russia

来源：

BIOORGANICHESKAYA KHIMIYA | 1999年 / 25卷 / 08期

关键词：

MALDI TOF mass spectrometry; protein expression in Escherichia coli; chimerical proteins;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The possible use of MALDI TOF MS for the analysis of Escherichia coli strains producing recombinant proteins was studied. It was shown that the target chimerical proteins might be rapidly detected in the strains.

引用

页码：638 / 640

页数：3

共 6 条

[1] PRINCIPLES AND INSTRUMENTATION IN TIME-OF-FLIGHT MASS-SPECTROMETRY - PHYSICAL AND INSTRUMENTAL CONCEPTS [J].

GUILHAUS, M .

JOURNAL OF MASS SPECTROMETRY, 1995, 30 (11) :1519-1532

[2] A THIOREDOXIN GENE FUSION EXPRESSION SYSTEM THAT CIRCUMVENTS INCLUSION BODY FORMATION IN THE ESCHERICHIA-COLI CYTOPLASM [J].

LAVALLIE, ER ;

DIBLASIO, EA ;

KOVACIC, S ;

GRANT, KL ;

SCHENDEL, PF ;

MCCOY, JM .

BIO-TECHNOLOGY, 1993, 11 (02) :187-193

[3]

Wang ZP, 1998, RAPID COMMUN MASS SP, V12, P456, DOI 10.1002/(SICI)1097-0231(19980430)12:8<456::AID-RCM177>3.0.CO

[4]

2-U

[5]

Welham KJ, 1998, RAPID COMMUN MASS SP, V12, P176, DOI 10.1002/(SICI)1097-0231(19980227)12:4<176::AID-RCM132>3.0.CO

[6]

2-T

← 1 →