Absolute levels of transcripts for mitochondrial uncoupling proteins UCP2, UCP3, UCP4, and UCP5 show different patterns in rat and mice tissues

被引：54

作者：

Alan, Lukas ^{[1
]}

Smolkova, Katarina ^{[1
]}

Kronusova, Eva ^{[1
]}

Santorova, Jitka ^{[1
]}

Jezek, Petr ^{[1
]}

机构：

[1] Acad Sci Czech Republ, Inst Physiol, Dept Membrane Transport Biophys, Inst Physiol, CR-14220 Prague 4, Czech Republic

来源：

JOURNAL OF BIOENERGETICS AND BIOMEMBRANES | 2009年 / 41卷 / 01期

关键词：

Mitochondrial uncoupling proteins; UCP2; UCP3; UCP4; UCP5; Absolute mRNA levels; POLYUNSATURATED FATTY-ACIDS; BROWN ADIPOSE-TISSUE; OXIDATIVE STRESS; SKELETAL-MUSCLE; IN-VIVO; EXPRESSION; CELLS; BRAIN; MEMBRANE; TRANSLATION;

D O I：

10.1007/s10863-009-9201-2

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Existing controversies led us to analyze absolute mRNA levels of mitochondrial uncoupling proteins (UCP1-UCP5). Individual UCP isoform mRNA levels varied by up to four orders of magnitude in rat and mouse tissues. UCP2 mRNA content was relatively high (0.4 to 0.8 pg per 10 ng of total mRNA) in rat spleen, rat and mouse lung, and rat heart. Levels of the same order of magnitude were found for UCP3 mRNA in rat and mouse skeletal muscle, for UCP4 and UCP5 mRNA in mouse brain, and for UCP2 and UCP5 mRNA in mouse white adipose tissue. Significant differences in pattern were found for rat vs. mouse tissues, such as the dominance of UCP3/UCP5 vs. UCP2 transcript in mouse heart and vice versa in rat heart; or UCP2 (UCP5) dominance in rat brain contrary to 10-fold higher UCP4 and UCP5 dominance in mouse brain. We predict high antioxidant/antiapoptotic UCP function in tissues with higher UCP mRNA content.

引用

页码：71 / 78

页数：8

共 46 条

[1] Mitochondrial uncoupling protein 1 expression in thymocytes [J].

Adams, Alison E. ;

Carroll, Audrey M. ;

Fallon, Padraic G. ;

Porter, Richard K. .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 2008, 1777 (7-8) :772-776

[2] Images of mitochondrial UCP 1 in mouse thymocytes using confocal microscopy [J].

Adams, Alison E. ;

Hanrahan, Orla ;

Nolan, Derek N. ;

Voorheis, H. Paul ;

Fallon, Padraic ;

Porter, Richard K. .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 2008, 1777 (02) :115-117

[3]

Affourtit Charles, 2007, Novartis Found Symp, V287, P70

[4] Disruption of the uncoupling protein-2 gene in mice reveals a role in immunity and reactive oxygen species production [J].

Arsenijevic, D ;

Onuma, H ;

Pecqueur, C ;

Raimbault, S ;

Manning, BS ;

Miroux, B ;

Couplan, E ;

Alves-Guerra, MC ;

Goubern, M ;

Surwit, R ;

Bouillaud, F ;

Richard, D ;

Collins, S ;

Ricquier, D .

NATURE GENETICS, 2000, 26 (04) :435-439

[5] Polyunsaturated fatty acids activate human uncoupling proteins 1 and 2 in planar lipid bilayers [J].

Beck, Valeri ;

Jaburek, Martin ;

Demina, Tatiana ;

Rupprecht, Anne ;

Porter, Richard K. ;

Jezek, Petr ;

Pohl, Elena E. .

FASEB JOURNAL, 2007, 21 (04) :1137-1144

[6] Regulation of mitochondrial uncoupling respiration during exercise in rat heart: Role of reactive oxygen species (ROS) and uncoupling protein 2 [J].

Bo, Hai ;

Jiang, Ning ;

Ma, Guodong ;

Qu, Jinting ;

Zhang, Guizhong ;

Cao, Dongning ;

Wen, Li ;

Liu, Shusen ;

Ji, Li Li ;

Zhang, Yong .

FREE RADICAL BIOLOGY AND MEDICINE, 2008, 44 (07) :1373-1381

[7] Uncoupling protein-3: A new member of the mitochondrial carrier family with tissue-specific expression [J].

Boss, O ;

Samec, S ;

PaoloniGiacobino, A ;

Rossier, C ;

Dulloo, A ;

Seydoux, J ;

Muzzin, P ;

Giacobino, JP .

FEBS LETTERS, 1997, 408 (01) :39-42

[8] Uncoupling proteins: A role in protection against reactive oxygen species - or not? [J].

Cannon, Barbara ;

Shabalina, Irina G. ;

Kramarova, Tatiana V. ;

Petrovic, Natasa ;

Nedergaard, Jan .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 2006, 1757 (5-6) :449-458

[9] Identification of a functioning mitochondrial uncoupling protein 1 in thymus [J].

Carroll, AM ;

Haines, LR ;

Pearson, TW ;

Fallon, PG ;

Walsh, CM ;

Brennan, CM ;

Breen, EP ;

Porter, RK .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (16) :15534-15543

[10] Mitochondrial uncoupling protein-4 regulates calcium homeostasis and sensitivity to store depletion-induced apoptosis in neural cells [J].

Chan, Sic. L. ;

Liu, Dong ;

Kyriazis, George A. ;

Bagsiyao, Pamela ;

Ouyang, Xin ;

Mattson, Mark P. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (49) :37391-37403

← 1 2 3 4 5 →