Induction of somatic hypermutation in immunoglobulin genes is dependent on DNA polymerase iota

被引：167

作者：

Faili, A ^{[1
]}

Aoufouchi, S ^{[1
]}

Flatter, E ^{[1
]}

Guéranger, Q ^{[1
]}

Reynaud, CA ^{[1
]}

Weill, JC ^{[1
]}

机构：

[1] Hop Necker Enfants Malad, INSERM, U373, F-75730 Paris 15, France

来源：

NATURE | 2002年 / 419卷 / 6910期

关键词：

D O I：

10.1038/nature01117

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Somatic hypermutation of immunoglobulin genes is a unique, targeted, adaptive process. While B cells are engaged in germinal centres in T-dependent responses, single base substitutions are introduced in the expressed V-H/V-L genes to allow the selection of mutants with a higher affinity for the immunizing antigen. Almost every possible DNA transaction has been proposed to explain this process, but each of these models includes an error-prone DNA synthesis step that introduces the mutations(1,2). The Y family of DNA polymerases(3)-pol eta, pol iota, pol kappa and rev1-are specialized for copying DNA lesions and have high rates of error when copying a normal DNA template(4,5). By performing gene inactivation in a Burkitt's lymphoma cell line inducible for hypermutation, we show here that somatic hypermutation is dependent on DNA polymerase iota.

引用

页码：944 / 947

页数：5

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