Gibberellins in seedlings and flowering trees of Prunus avium L.

Extracts of acids from mature seeds, germinating seeds, first, second and third year seedlings as well as mature, flowering trees of sweet cherry (Prunus avium L. cv. Stella) were analysed by gas chromatography-mass spectrometry. The presence of the known gibberellins (GAs) GA(1) (1), GA(3) (4), GA(5) (7), GA(8) (11), GA(19) (14), GA(20) (12), GA(29) (13), GA(32) (5), GA(85) (2), GA(86) (3) and GA(87) (6) was confirmed by comparison of their mass spectra and Kovats retention indices with those of standards or literature values. In addition, 16 alpha,17-dihydrodihydroxy GA(25) (16) was identified and its stereochemistry confirmed by rational synthesis. The 12 alpha,13-dihydroxy GAs, GA(32) (5), GA(85) (2), GA(86) (3) and GA(87) (6), were detected in mature seeds, germinating seeds and young seedlings, but not in flowering plants. The 13-hydroxy GAs, GA(1) (1) and GA(3) (4), were present in germinating seeds and, in addition to these, GA(5) (7), GA(8) (11), GA(19) (14), GA(20) (12) and GA(29) (13) were detected in seedlings and mature flowering plants. In germinating seeds and seedlings (while the plants were growing actively), concentrations of the 12 alpha,13-dihydroxy GAs, measured by bioassay, declined and those of the 13-hydroxy GAs increased. The results are discussed with reference to the known and predicted effects of the GAs on the vegetative growth and flowering of P. avium plants. (C) 2000 Elsevier Science Ltd. All rights reserved.