Separation and determination of glycolipids from edible plant sources by high-performance liquid chromatography and evaporative light-scattering detection

被引：164

作者：

Sugawara, T ^{[1
]}

Miyazawa, T ^{[1
]}

机构：

[1] Tohoku Univ, Grad Sch Life Sci & Agr, Chem Biodynam Lab, Sendai, Miyagi 9818555, Japan

来源：

LIPIDS | 1999年 / 34卷 / 11期

关键词：

D O I：

10.1007/s11745-999-0476-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glycolipids from edible plant sources were accurately quantified by silica-based, normal-phase high-performance liquid chromatography using an evaporative light-scattering detector. Five major glycolipid classes (acylated steryl glucoside, steryl glucoside, ceramide monohexoside, monogalactosyldiacylglycerol, and digalactosyldiacylglycerol) were separated and determined with a binary gradient system consisting of chloroform and methanol/water (95:5, vol/vol) without any interference from other lipid classes and pigments. The described method was applied to 48 edible plants available in Japan including cereals, legumes, vegetables, and fruits. Examined plant species contained glycolipids in wide concentration ranges, such as 5-645 mg/100 g tissue.

引用

页码：1231 / 1237

页数：7

共 38 条

[1]

ALLEN CF, 1966, J AM OIL CHEM SOC, V43, P223

[2]

ANDERSSON L, 1995, J LIPID RES, V36, P1392

[3] QUANTITATIVE-ANALYSIS OF PULMONARY SURFACTANT PHOSPHOLIPIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND LIGHT-SCATTERING DETECTION [J].