Discrepancy between the effects of morronside on apoptosis in human embryonic lung fibroblast cells and lung cancer A549 cells

被引：23

作者：

Chen, Jian-Ping ^{[1
]}

Xu, De-Guo ^{[1
]}

Yu, Xiao-Yi ^{[2
]}

Zhao, Feng-Ming ^{[1
]}

Xu, Dong-Qing ^{[1
]}

Zhang, Xu ^{[1
]}

Cai, Bao-Chang ^{[1
]}

Wang, Ming-Yan ^{[1
]}

机构：

[1] Nanjing Univ Chinese Med, Jiangsu Key Lab Chinese Med Proc, Nanjing 210029, Jiangsu, Peoples R China

[2] China Pharmaceut Univ, Nanjing 210009, Jiangsu, Peoples R China

来源：

ONCOLOGY LETTERS | 2014年 / 7卷 / 04期

关键词：

apoptosis; retinoblastoma protein; morroniside; human embryonic lung fibroblasts cells; CYCLE; PATHWAY;

D O I：

10.3892/ol.2014.1850

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Morroniside is a water-soluble compound extracted from the fruit of Cornus officinalis and is used to protect lung activity against aging. In the present study, the manner in which morroniside regulates normal lung and cancer cells was examined. The human embryonic lung fibroblast (HELF) cell line and lung cancer A549 cell line, and their responses to morroniside treatment, were examined. Results showed that morroniside reverses the apoptotic effect of H2O2 on HELF cell growth, protecting cell proliferation and normal cell morphology and inhibiting apoptosis. However, these effects were not present in A549 cells. Western blotting showed that morroniside also markedly downregulated retinoblastoma protein in HELF cells. These results suggest that morroniside treatment exhibits different effects on apoptosis in HELF and A549 cells, making it a viable compound for decreasing the side effects of anticancer medicines in normal cells.

引用

页码：927 / 932

页数：6

共 21 条

[1]

Ai Hou-Xi, 2008, Zhongguo Zhong Yao Za Zhi, V33, P2109

[2] Antioxidant systems and oxidative stress in the testes (Reprinted from Molecular Mechanisms in Spermatogenesis, 2007) [J].

Aitken, R. John ;

Roman, Shaun D. .

OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2008, 1 (01) :15-24

[3] The atypical E2F family member E2F7 couples the p53 and RB pathways during cellular senescence [J].

Aksoy, Ozlem ;

Chicas, Agustin ;

Zeng, Tianying ;

Zhao, Zhen ;

McCurrach, Mila ;

Wang, Xiaowo ;

Lowe, Scott W. .

GENES & DEVELOPMENT, 2012, 26 (14) :1546-1557

[4]

Antonsson A, 2009, ANTICANCER RES, V29, P2893

[5] Regulation of RB Transcription In Vivo by RB Family Members [J].

Burkhart, Deborah L. ;

Ngai, Lynn K. ;

Roake, Caitlin M. ;

Viatour, Patrick ;

Thangavel, Chellappagounder ;

Ho, Victoria M. ;

Knudsen, Erik S. ;

Sage, Julien .

MOLECULAR AND CELLULAR BIOLOGY, 2010, 30 (07) :1729-1745

[6] Conserved RB functions in development and tumor suppression [J].

Gordon, Gabriel M. ;

Du, Wei .

PROTEIN & CELL, 2011, 2 (11) :864-878

[7] Overexpression of cyclin E/CDK2 complexes overcomes FGF-induced cell cycle arrest in the presence of hypophosphorylated Rb proteins [J].

Kolupaeva, Victoria ;

Basilico, Claudio .

CELL CYCLE, 2012, 11 (13) :2557-2566

[8] PP1 and PP2A phosphatases - cooperating partners in modulating retinoblastoma protein activation [J].

Kolupaeva, Victoria ;

Janssens, Veerle .

FEBS JOURNAL, 2013, 280 (02) :627-643

[9] The Pharmacological Effects of Morroniside and Loganin Isolated from Liuweidihuang Wan, on MC3T3-E1 Cells [J].

Li, Manyu ;

Wang, Wei ;

Wang, Ping ;

Yang, Kun ;

Sun, Hui ;

Wang, Xijun .

MOLECULES, 2010, 15 (10) :7403-7414

[10]

Li XL, 2004, ZHONGGUO YAOSHI, V7, P767

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