Expanding the genetic code of Mus musculus

被引：69

作者：

Han, Songmi ^{[1
]}

Yang, Aerin ^{[2
]}

Lee, Soonjang ^{[1
]}

Lee, Han-Woong ^{[3
]}

Park, Chan Bae ^{[1
]}

Park, Hee-Sung ^{[2
]}

机构：

[1] Ajou Univ, Dept Physiol, Sch Med, Suwon 16499, South Korea

[2] Korea Adv Inst Sci & Technol, Dept Chem, 291 Daehak Ro, Daejeon 34141, South Korea

[3] Yonsei Univ, Dept Biochem, Yonsei Lab Anim Res Ctr, Seoul 03722, South Korea

来源：

NATURE COMMUNICATIONS | 2017年 / 8卷

基金：

新加坡国家研究基金会;

关键词：

MESSENGER-RNA DECAY; TRANSCRIPTION; CHEMISTRY; PROTEINS; BIOLOGY; CANCER; MTDNA;

D O I：

10.1038/ncomms14568

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Here we report the expansion of the genetic code of Mus musculus with various unnatural amino acids including Ne-acetyl-lysine. Stable integration of transgenes encoding an engineered Ne-acetyl-lysyl-tRNA synthetase (AcKRS)/tRNAPyl pair into the mouse genome enables site-specific incorporation of unnatural amino acids into a target protein in response to the amber codon. We demonstrate temporal and spatial control of protein acetylation in various organs of the transgenic mouse using a recombinant green fluorescent protein (GFPuv) as a model protein. This strategy will provide a powerful tool for systematic in vivo study of cellular proteins in the most commonly used mammalian model organism for human physiology and disease.

引用

页数：7

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