Long noncoding RNA expression patterns in lymph node metastasis in colorectal cancer by microarray

被引:13
作者
Rui, Qiang [1 ]
Xu, Zipeng [1 ]
Yang, Peng [1 ]
He, Zhenyu [1 ]
机构
[1] Nanjing Med Univ, Affiliated Hosp 2, Nanjing, Jiangsu, Peoples R China
关键词
Lymph node metastasis; Colorectal cancer; Long noncoding RNA; Microarray;
D O I
10.1016/j.biopha.2015.08.003
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Aim: To profile the long noncoding RNA (lncRNA) expression patterns in normal lymph node (NLN) of colorectal cancer compared with paired metastatic lymph node (MLN). Materials and methods: MLN, NLN lncRNA and messenger RNA (mRNA) expression levels were determined by microarray in three patients; quantitative reverse transcription-polymerase chain reaction validation of 3 differentially expressed lncRNAs was conducted in another 26 patients. The lncRNAs' functions were predicted through coexpressed mRNA annotations. Result(s): A total of 1133 lncRNA transcripts transcripts were dysregulated in metastatic lymph node, compared with normal lymph node. The expressions of the 3 chosen lncRNAs were validated by quantitative reverse transcription-polymerase chain reaction. Functional analysis suggests that several groups of lncRNAs may participate in biological pathways related to colorectal cancer by cis-and/or trans-regulation of protein-coding genes. Conclusion(s): This study constitutes the first report of lncRNA expression patterns in human MLN, NLN of colorectal cancer. More than 1000 dysregulated lncRNA transcripts are found by microarray. (C) 2015 Elsevier Masson SAS. All rights reserved.
引用
收藏
页码:12 / 18
页数:7
相关论文
共 21 条
[1]  
[Anonymous], CLIN TRANSL ONCOL
[2]  
[Anonymous], NATURE
[3]  
[Anonymous], WORLD J GASTROENTERO
[4]   Long Noncoding RNAs: Cellular Address Codes in Development and Disease [J].
Batista, Pedro J. ;
Chang, Howard Y. .
CELL, 2013, 152 (06) :1298-1307
[5]   Long Noncoding RNAs: New Players in the Molecular Mechanism for Maintenance and Differentiation of Pluripotent Stem Cells [J].
Ghosal, Suman ;
Das, Shaoli ;
Chakrabarti, Jayprokas .
STEM CELLS AND DEVELOPMENT, 2013, 22 (16) :2240-2253
[6]   The Trop-2 signalling network in cancer growth [J].
Guerra, E. ;
Trerotola, M. ;
Aloisi, A. L. ;
Tripaldi, R. ;
Vacca, G. ;
La Sorda, R. ;
Lattanzio, R. ;
Piantelli, M. ;
Alberti, S. .
ONCOGENE, 2013, 32 (12) :1594-1600
[7]   lincRNAs act in the circuitry controlling pluripotency and differentiation [J].
Guttman, Mitchell ;
Donaghey, Julie ;
Carey, Bryce W. ;
Garber, Manuel ;
Grenier, Jennifer K. ;
Munson, Glen ;
Young, Geneva ;
Lucas, Anne Bergstrom ;
Ach, Robert ;
Bruhn, Laurakay ;
Yang, Xiaoping ;
Amit, Ido ;
Meissner, Alexander ;
Regev, Aviv ;
Rinn, John L. ;
Root, David E. ;
Lander, Eric S. .
NATURE, 2011, 477 (7364) :295-U60
[8]   Chromatin signature reveals over a thousand highly conserved large non-coding RNAs in mammals [J].
Guttman, Mitchell ;
Amit, Ido ;
Garber, Manuel ;
French, Courtney ;
Lin, Michael F. ;
Feldser, David ;
Huarte, Maite ;
Zuk, Or ;
Carey, Bryce W. ;
Cassady, John P. ;
Cabili, Moran N. ;
Jaenisch, Rudolf ;
Mikkelsen, Tarjei S. ;
Jacks, Tyler ;
Hacohen, Nir ;
Bernstein, Bradley E. ;
Kellis, Manolis ;
Regev, Aviv ;
Rinn, John L. ;
Lander, Eric S. .
NATURE, 2009, 458 (7235) :223-227
[9]   Global Cancer Statistics [J].
Jemal, Ahmedin ;
Bray, Freddie ;
Center, Melissa M. ;
Ferlay, Jacques ;
Ward, Elizabeth ;
Forman, David .
CA-A CANCER JOURNAL FOR CLINICIANS, 2011, 61 (02) :69-90
[10]   Epigenetic Regulation by Long Noncoding RNAs [J].
Lee, Jeannie T. .
SCIENCE, 2012, 338 (6113) :1435-1439