Effects of sodium arsenite on catalase activity, gene and protein expression in HaCaT cells

被引：25

作者：

Sun, Xiance

Li, Bing

Li, Xin

Wang, Yi

Xu, Yuanyuan

Jin, Yaping

Piao, Fengyuan

Sun, Guifan ^{[1
]}

机构：

[1] China Med Univ, Sch Publ Hlth, Dept Environm & Occupat Hlth, Shenyang, Liaoning, Peoples R China

[2] Dalian Med Univ, Coll Basic Med Sci, Dept Hyg, Dalian, Liaoning, Peoples R China

来源：

TOXICOLOGY IN VITRO | 2006年 / 20卷 / 07期

基金：

中国国家自然科学基金;

关键词：

arsenite; oxidative stress; catalase;

D O I：

10.1016/j.tiv.2006.02.008

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

The objective of this research work is to study the effects of sodium arsenite on activity, mRNA and protein expression of catalase (CAT) in established human cell lines of keratinocytes (HaCaT). Based on the AlamarBlue assay, we found that a high level (100 mu mol/l) of sodium arsenite inhibited cell viability. The reactive oxygen species (ROS) content in cells was increased in all treated cultures dose-dependently. CAT activity, mRNA expression and protein levels were decreased by 5-20 mu mol/l of sodium arsenite. It is highly suggestive that CAT gene expression and protein levels are affected by arsenic. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：1139 / 1144

页数：6

共 24 条

[1] Arsenic: Health effects, mechanisms of actions, and research issues [J].