Detection of Mycobacterium tuberculosis complex organisms in the stools of patients with pulmonary tuberculosis

被引:59
作者
El Khechine, Amel [1 ]
Henry, Mireille [1 ]
Raoult, Didier [1 ]
Drancourt, Michel [1 ]
机构
[1] Univ Mediterranee & Pole Malad Infect, Assistance Publ Hop Marseille, Fac Med, URMITE,CNRS,IRD,UMR 6236,IFR48, Marseille, France
来源
MICROBIOLOGY-SGM | 2009年 / 155卷
关键词
AVIUM COMPLEX; DIAGNOSIS; CULTURE; RECOVERY; SPUTUM; SMEAR; IDENTIFICATION; SPECIMENS; CHILDREN; ASSAY;
D O I
10.1099/mic.0.026484-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The laboratory diagnosis of pulmonary tuberculosis mainly relies on the detection of Mycobacterium tuberculosis complex (MTC) organisms in the sputum. In patients who do not give sputum, alternative respiratory tract specimens can be obtained only by invasive procedures. Based on the known survival of MTC organisms in the gastric fluid, we hypothesized that swallowed MTC organisms would be detectable in stool samples. We compared the presence of MTC organisms in respiratory tract specimens and stool specimens collected in parallel from the same patients. MTC was detected in cultures grown on egg-based medium after appropriate decontamination, by microscopic examination after Ziehl-Neelsen staining and by real-time PCR detection of IS6110 using internal controls. A case of pulmonary tuberculosis was defined by the presence of (i) clinical and radiological signs and symptoms suggestive of pulmonary tuberculosis, and (ii) culture of MTC organisms from at least one respiratory tract specimen or (iii) the presence of acid-fast bacilli in the sputum that were subsequently identified as MTC organisms by real-time PCR. The observation of 134 patients suspected to be suffering pulmonary tuberculosis led to the identification of 24 cases and 110 non-infected control patients. Cases and controls did not significantly differ with respect to sex but cases were significantly younger than controls. The sensitivity/specificity was 37.5%/100% for the microscopic examination of stools, 54.2%/100% for culturing and 100%/97.3% for real-time PCR. The positive predicted value was 100%, 100% and 88.9%, respectively, and the negative predicted value was 88%, 90.9% and 100%,respectively. In four patients, a stool specimen initially yielded the correct diagnosis of pulmonary tuberculosis before evaluation of the respiratory tract specimen confirmed the diagnosis. These data indicate that stools could be used in conjunction with sputum testing or as an alternative specimen upon which to base the diagnosis of pulmonary tuberculosis by molecular identification of acid-fast bacilli and culture. This non-invasive alternative procedure is of particular interest for patients who cannot expectorate.
引用
收藏
页码:2384 / 2389
页数:6
相关论文
共 35 条
[1]   Microbiological diagnostic procedures in respiratory infections: mycobacterial infections [J].
Andresen, David .
PAEDIATRIC RESPIRATORY REVIEWS, 2007, 8 (03) :221-230
[2]   EFFICACIES OF SELECTED DISINFECTANTS AGAINST MYCOBACTERIUM-TUBERCULOSIS [J].
BEST, M ;
SATTAR, SA ;
SPRINGTHORPE, VS ;
KENNEDY, ME .
JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (10) :2234-2239
[3]   Determination of HPV type 16 and 18 viral load in cervical smears of women referred to colposcopy [J].
Carcopino, X. ;
Henry, M. ;
Benmoura, D. ;
Fallabregues, A. S. ;
Richet, H. ;
Boubli, L. ;
Tamalet, C. .
JOURNAL OF MEDICAL VIROLOGY, 2006, 78 (08) :1131-1140
[4]   Tuberculosis in children and adolescents, Taiwan, 1996-2003 [J].
Chan, Pei-Chun ;
Huang, Li-Min ;
Wu, Yi-Chun ;
Yang, Hsiang-Lin ;
Chang, I-Shou ;
Lu, Chun-Yi ;
Lee, Ping-Ing ;
Lee, Chin-Yun ;
Chang, Luan-Yin .
EMERGING INFECTIOUS DISEASES, 2007, 13 (09) :1361-1363
[5]   Diagnostic value of gastric aspirate smear and polymerase chain reaction in smear-negative pulmonary tuberculosis [J].
Chierakul, N ;
Anantasetagoon, T ;
Chaiprasert, A ;
Tingtoy, N .
RESPIROLOGY, 2003, 8 (04) :492-496
[6]  
COLEBUNDERS R, 1990, ANN SOC BELG MED TR, V70, P303
[7]   Early diagnosis of disseminated Mycobacterium genavense infection [J].
de Lastours, Victoire ;
Guillemain, Romain ;
Mainardi, Jean-Luc ;
Aubert, Agnes ;
Chevalier, Patrick ;
Lefort, Agnes ;
Podglajen, Isabelle .
EMERGING INFECTIOUS DISEASES, 2008, 14 (02) :346-347
[8]   The Role of Flexible Bronchoscopy in Pediatric Pulmonary Tuberculosis [J].
Dey, Shuvra Kanti ;
Islam, Abul ;
Mizuguchi, Masashi ;
Okitsu, Shoko ;
Ushijima, Hiroshi .
JOURNAL OF TROPICAL PEDIATRICS, 2008, 54 (06) :423-425
[9]   A Single-Step Sequencing Method for the Identification of Mycobacterium tuberculosis Complex Species (Publication with Expression of Concern) [J].
Djelouadji, Zoheira ;
Raoult, Didier ;
Daffe, Mamadou ;
Drancourt, Michel .
PLOS NEGLECTED TROPICAL DISEASES, 2008, 2 (06)
[10]   Stool microscopy and culture to assist the diagnosis of pulmonary tuberculosis in childhood [J].
Donald, PR ;
Schaaf, HS ;
Gie, RP ;
Beyers, N ;
Sirgel, FA ;
Venter, A .
JOURNAL OF TROPICAL PEDIATRICS, 1996, 42 (05) :311-312