Detection of Borrelia burgdorferi, Anaplasma phagocytophilum and Babesia microti, with two different multiplex PCR assays

被引:68
作者
Hojgaard, Andrias [1 ]
Lukacik, Gary [2 ]
Piesman, Joseph [1 ]
机构
[1] Ctr Dis Control & Prevent, Div Vector Borne Dis, Natl Ctr Emerging & Zoonot Infect Dis, Ft Collins, CO 80521 USA
[2] New York State Dept Hlth, Bur Communicable Dis Control, Albany, NY USA
关键词
Multiplex PCR; Ticks; Pathogens; TICKS;
D O I
10.1016/j.ttbdis.2013.12.001
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
We have developed 2 real-time multiplex PCR assays for detection of Borrelia burgdorferi, Anaplasma phagocytophilum, and Babesia microti. The efficiency and sensitivity of each multiplex PCR assay was evaluated using field-collected Ixodes scapularis ticks that were positive for each of the pathogens, cloned plasmids harboring each of the PCR targets, and laboratory I. scapularis infected with B. burgdorferi B31. There was no difference in efficiency or sensitivity when comparing the multiplex PCR with the individual PCR reactions. If the 2 multiplex PCR assays are used in the same analysis, field-collected ticks that only harbor B. miyamotoi can also be identified. The multiplex assays are fast and cost-effective methods for screening and detecting pathogens in ticks, when compared to single-target PCR. Published by Elsevier GmbH.
引用
收藏
页码:349 / 351
页数:3
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