Diamine oxidase as a marker of intestinal mucosal injury and the effect of soluble dietary fiber on gastrointestinal tract toxicity after intravenous 5-fluorouracil treatment in rats

被引：175

作者：

Fukudome, Ian ^{[1
]}

Kobayashi, Michiya ^{[1
,2
]}

Dabanaka, Ken ^{[1
]}

Maeda, Hiromichi ^{[1
,2
]}

Okamoto, Ken ^{[1
,2
]}

Okabayashi, Takehiro ^{[1
]}

Baba, Ryoko ^{[3
]}

Kumagai, Nana ^{[3
]}

Oba, Koji ^{[4
]}

Fujita, Mamoru ^{[5
]}

Hanazaki, Kazuhiro ^{[1
]}

机构：

[1] Kochi Med Sch, Dept Surg, Kochi 7838505, Japan

[2] Kochi Med Sch, Dept Human Hlth & Med Sci, Kochi 7838505, Japan

[3] Univ Occupat & Environm Hlth, Sch Med, Dept Anat, Kitakyushu, Fukuoka 807, Japan

[4] Hokkaido Univ Hosp, Translat Res & Clin Trial Ctr, Sapporo, Hokkaido 060, Japan

[5] Nakamura Gakuen, Grad Sch Hlth & Nutr Sci, Fukuoka, Japan

来源：

MEDICAL MOLECULAR MORPHOLOGY | 2014年 / 47卷 / 02期

关键词：

Diarrhea; DAO; Chemotherapy; Side effect; Histaminase; SDF; CELL-PROLIFERATION; HISTAMINASE; DIARRHEA;

D O I：

10.1007/s00795-013-0055-7

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The level of plasma diamine oxidase (DAO) activity is associated with the maturation and integrity of small intestinal mucosa. This study in rats investigated whether a decreased level of plasma DAO could reflect the severity of mucosal injury due to intravenous 5-fluorouracil (5-FU) treatment. The beneficial effect of soluble dietary fiber (SDF) on preventing diarrhea after 5-FU treatment was also examined. To induce diarrhea, 5-FU (50 mg/kg/day for four days) was administered via the tail vein with or without SDF supplementation. After 5-FU treatment, the majority of rats developed moderate to severe diarrhea, and levels of plasma DAO activity significantly decreased compared to those of control group (P < 0.05). Scanning electron microscopy revealed disarrangement of the small intestinal villi. Contrarily, the rats supplemented with SDF had diarrhea less frequently (50.0 vs. 91.7 %, P = 0.025) on day five, and DAO activity levels were significantly higher than in those rats administered 5-FU alone (8.25 +/- A 5.34 vs. 5.50 +/- A 4.32, P = 0.023). In conclusion, plasma DAO activity decreases in response to severe intestinal mucosal injury after 5-FU treatment, and SDF supplementation might be a practical and useful treatment for reducing the intestinal toxicity of 5-FU.

引用

页码：100 / 107

页数：8

共 18 条

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