Antioxidant and anti-inflammatory activities of Prussian blue nanozyme promotes full-thickness skin wound healing

被引:104
作者
Sahu, Abhishek [1 ]
Jeon, Jin [2 ,3 ]
Lee, Min Suk [2 ,3 ]
Yang, Hee Seok [2 ,3 ,4 ]
Tae, Giyoong [1 ]
机构
[1] Gwangju Inst Sci & Technol, Sch Mat Sci & Engn, Gwangju 61005, South Korea
[2] Dankook Univ, Dept Nanobiomed Sci, Cheonan 31116, South Korea
[3] Dankook Univ, BK21 PLUS NBM Global Res Ctr Regenerat Med, Cheonan 31116, South Korea
[4] Dankook Univ, Ctr Biomed Engn Core Facil, Cheonan 31116, South Korea
来源
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2021年 / 119卷
基金
新加坡国家研究基金会;
关键词
Nanozyme; Prussian blue; ROS scavenging; Anti-inflammatory; Wound healing; CERIUM OXIDE NANOPARTICLES; OXIDATIVE STRESS; TOXICITY; AGENT; LIPOPOLYSACCHARIDE; INTERVENTIONS; SILVER; DNA;
D O I
10.1016/j.msec.2020.111596
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
0805 ; 080501 ; 080502 ;
摘要
Excessive reactive oxygen species (ROS) and unresolved inflammations are the major causes of impaired wound healing as they overwhelm the cellular antioxidant system and impede the healing process. In this study, we examined the application of Prussian blue (PB) nanozyme as a novel material for cutaneous wound healing through the alleviation of excessive ROS and inflammation modulation. The PB nanoparticles not only exhibited hydrogen peroxide (H2O2) degradation activity but also showed strong superoxide scavenging ability. PB nanozyme mitigated the intracellular ROS at a high oxidative stress environment, resulting in a pronounced cytoprotective effect. Moreover, PB nanozyme also displayed significant anti-inflammatory activity, as evident from the suppression of inflammatory mediators in the lipopolysaccharide (LPS) induced macrophage cells. Encouraged by the in vitro results, we evaluated the in vivo therapeutic efficacy of PB nanozyme in a full thickness cutaneous wound model combined with LPS treatment to mimic bacterial infection. The beneficial effects of topically applied PB nanozyme on wound healing and tissue regeneration were evident compared to the control. The periodical administration of a low amount (50 mu g x 4) of PB nanoparticles exhibited faster wound closure as well as collagen deposition, maturation, and organization. Moreover, the PB treatment effectively induced the differentiation of keratinocytes, enhanced the neovascularization, and reduced macrophage burden in the entire wound site. Thus, PB nanozyme not only accelerated the healing process in an infection-mimicking cutaneous wound model but also exhibited tissue regeneration characteristics owing to the synergistic effect of ROS-scavenging and anti-inflammatory activities.
引用
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页数:14
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