Changes in the antioxidative systems in mitochondria during ripening of pepper fruits

被引：45

作者：

Jiménez, A ^{[1
]}

Gómez, JM ^{[1
]}

Navarro, E ^{[1
]}

Sevilla, F ^{[1
]}

机构：

[1] CSIC, Ctr Edafol & Biol Aplicada Segura, Nutr & Plant Physiol Dept, Murcia 30080, Spain

来源：

PLANT PHYSIOLOGY AND BIOCHEMISTRY | 2002年 / 40卷 / 6-8期

关键词：

ascorbate; glutathione; mitochondria; pepper fruit; ripening;

D O I：

10.1016/S0981-9428(02)01424-9

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The presence of enzymes of the ascorbate-glutathione cycle was studied in mitochondria purified from green and red pepper (Capsicum annuum L.) fruits. All four enzymes, ascorbate peroxidase (APX; EC 1.11.1.11), monodehydroascorbate reductase (MDHAR; EC 1.6.5.4), dehydroascorbate reductase (DHAR; EC 1.8.5.1) and glutathione reductase (GR; EC 1.6.4.2) were present in the isolated mitochondria of both fruit ripening stages. The activity of the reductive ascorbate-glutathione cycle enzymes (MDHAR, GR and DHAR) was higher in mitochondria isolated from green than from red fruits, while APX and the antioxidative enzyme superoxide dismutase (SOD; EC 1.15.1.1) were higher in the red fruits. The levels of ascorbate and L-galactono-gamma-lactone dehydrogenase (GLDH; EC 1.3.2.3) activity were found to be similar in the mitochondria of both fruits. The higher APX and Mn-SOD specific activities in mitochondria from red fruits might play a role in avoiding the accumulation of any activated oxygen species generated in these mitochondria, and suggests an active role for these enzymes during ripening. 0 2002 Editions scientifiques et medicales Elsevier SAS. All rights reserved.

引用

页码：515 / 520

页数：6

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