Proteomic studies of the intrinsically unstructured mammalian proteome

被引：53

作者：

Galea, Charles A.

Pagala, Vishwajeeth R.

Obenauer, John C.

Park, Cheon-Gil

Slaughter, Clive A.

Kriwacki, Richard W.

机构：

[1] St Jude Childrens Res Hosp, Dept Biol Struct, Memphis, TN 38105 USA

[2] St Jude Childrens Res Hosp, Hartwell Ctr Bioinformat & Biotechnol, Memphis, TN 38105 USA

[3] Univ Tennessee, Ctr Hlth Sci, Dept Mol Sci, Memphis, TN 38163 USA

来源：

JOURNAL OF PROTEOME RESEARCH | 2006年 / 5卷 / 10期

关键词：

intrinsically unstructured proteins; disordered proteins; proteomics; mammalian proteome; heat denaturation;

D O I：

10.1021/pr060328c

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Intrinsically unstructured proteins (IUPs) represent an important class of proteins primarily involved in cellular signaling and regulation. The aim of this study was to develop methodology for the enrichment and identification of IUPs. We show that heat treatment of NIH3T3 mouse fibroblast cell extracts at 98 C selects for IUPs. The majority of these IUPs were cytosolic or nuclear proteins involved in cell signaling or regulation. These studies represent the first large-scale experimental investigation of the intrinsically unstructured mammalian proteome.

引用

页码：2839 / 2848

页数：10

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