Apelin-13 attenuates motor impairments and prevents the changes in synaptic plasticity-related molecules in the striatum of Parkinsonism rats

被引：21

作者：

Haghparast, Elham ^{[1
,2
]}

Sheibani, Vahid ^{[1
]}

Abbasnejad, Mehdi ^{[2
]}

Esmaeili-Mahani, Saeed ^{[1
,2
]}

机构：

[1] Kerman Univ Med Sci, Inst Neuropharmacol, Kerman Neurosci Res Ctr, Lab Mol Neurosci, Kerman, Iran

[2] Shahid Bahonar Univ Kerman, Fac Sci, Dept Biol, POB 76135-133, Kerman, Iran

来源：

PEPTIDES | 2019年 / 117卷

基金：

美国国家科学基金会;

关键词：

Apelin-13; Parkinson's disease; 6-OHDA; Motor impairment; Striatum; Synaptic plasticity; METABOTROPIC GLUTAMATE RECEPTORS; MOUSE MODEL; POSTSYNAPTIC DENSITY-95; APJ RECEPTOR; PSD-95; PEPTIDE; PROTEIN; MODULATION; EXPRESSION; LIGAND;

D O I：

10.1016/j.peptides.2019.05.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The striatum plays a critical role in motor control and also learning and memory of motor skills. It has been reported that striatal synaptic components are significantly decreased in dopaminergic-denervated striatum. In this study the effects of apelin-13 were investigated on motor disorders and striatal synaptosomal expression of PSD-95, neurexin1, neuroligin, metabotropic glutamate receptor (mGlu R1) and dopaminergic receptors (DR1 and DR2) in rat parkinsonism experimental model. 6-hydroxydopamine (6-OHDA) was injected into the substantia nigra. Apelin-13 (1, 2 and 3 mu g/rat) was administered into the substantia nigra one week after the 6-OHDA injection. Accelerating rotarod, beam-balance, beam-walking and bar tests were performed one month after the apelin injection. Immunohistochemistry staining of dopaminergic neurons was performed. The levels of synaptic proteins were determined by immunoblotting. 6-OHDA-treated animals showed a significant impairment in motor-skill tasks and a dramatically change in the expression levels of mentioned proteins. Apelin-13 (3 mu g/rat) significantly attenuates the motor impairments and prevents the changes in striatal synaptic elements in 6-OHDA-treated animals. In addition, it could rescue the dopaminergic neurons of the substantia nigra. The data will potentially extend the possible benefic aspect of apelin in neurodegenerative disorders.

引用

页数：9

共 53 条

[1] Fingolimod modulates multiple neuroinflammatory markers in a mouse model of Alzheimer's disease [J].