Splicing of a Bacterial Group II Intron from Bacillus megaterium Is Independent of Intron-Encoded Protein

被引：2
作者：
Chien, Mei-Fang [1 ,2 ]
Tosa, Saeko [1 ]
Huang, Chieh-Chen [3 ]
Endo, Ginro [1 ]
机构：
[1] Tohoku Gakuin Univ, Fac Engn, Tagajo, Miyagi 9858537, Japan
[2] Tohoku Univ, Grad Sch Life Sci, Aoba Ku, Sendai, Miyagi 9808577, Japan
[3] Natl Chung Hsing Univ, Dept Life Sci, Taichung 40227, Taiwan
来源：
MICROBES AND ENVIRONMENTS | 2009年 / 24卷 / 01期
关键词：
group II intron; intron-encoded protein; splicing; MATURASE BINDING; RNA; IDENTIFICATION; DIVERSITY; SEDIMENT; DOMAIN; DNA;
D O I：
10.1264/jsme2.ME08540
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The splicing of a bacterial group II subclass B intron B.me.I1 from Bacillus megaterium chromosomes was investigated. RT-PCR and nucleic acid hybridization methods were used to understand the role of the intron-encoded protein (IEP) in the splicing of B.me.I1. An in vivo assay showed that the splicing occurred in the absence of IEP. An in vitro assay showed that B.me.I1 was spliced under conditions similar to those of the intracellular environment with no help from other biological molecules. Because all group II introns previously reported needed IEPs for their splicing in vivo, our results suggest that B.me.I1 is an "actual" self-splicing group II intron. This is also the first report to recognize the existence of group II introns that independently splice mRNA in vivo. The self-splicing of a bacterial intron may support that eukaryotic spliceosomal introns originated in bacterial genomes.
引用
收藏
页码：28 / 32
页数：5
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