LncRNA-ATB promotes TGF-β-induced glioma cells invasion through NF-κB and P38/MAPK pathway

被引：79

作者：

Tang, Feng ^{[1
,2
]}

Wang, Hongliang ^{[1
,2
]}

Chen, Erfeng ^{[1
,2
]}

Bian, Erbao ^{[1
,2
]}

Xu, Yadi ^{[1
]}

Ji, Xinghu ^{[1
,2
]}

Yang, Zhihao ^{[1
,2
]}

Hua, Xiangyang ^{[1
,2
]}

Zhang, Yile ^{[1
,2
]}

Zhao, Bing ^{[1
,2
]}

机构：

[1] Anhui Med Univ, Dept Neurosurg, Affiliated Hosp 2, Hefei, Anhui, Peoples R China

[2] Anhui Med Univ, Cerebral Vasc Dis Res Ctr, Hefei, Anhui, Peoples R China

来源：

JOURNAL OF CELLULAR PHYSIOLOGY | 2019年 / 234卷 / 12期

基金：

中国国家自然科学基金;

关键词：

glioma; invasion; lncRNA-ATB; NF-kappa B; P38; MAPK; TGF-beta; P38; MAPK; GROWTH; RNA; ACTIVATION; CANCER; APOPTOSIS; RECEPTOR; PROGRESSION; PROLIFERATION; GLIOBLASTOMA;

D O I：

10.1002/jcp.28898

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Glioma constitutes the most aggressive primary intracranial malignancy in adults. We previously showed that long noncoding RNA activated by TGF-beta (lncRNA-ATB) promoted the glioma cells invasion. However, whether lncRNA-ATB is involved in TGF-beta-mediated invasion of glioma cells remains unknown. In this study, quantitative real-time polymerase chain reaction and western blot analysis were used for detecting the mRNA and protein expression of related genes, respectively. Transwell assay was performed to assess the impact of lncRNA-ATB on TGF-beta-induced glioma cells migration and invasion. Immunofluorescence staining was utilized to characterize related protein distribution. Results showed that TGF-beta upregulated lncRNA-ATB expression in glioma LN-18 and U251 cells. Overexpression of lncRNA-ATB activated nuclear factor-kappa B (NF-kappa B) pathway and promoted P65 translocation into the nucleus, thus facilitated glioma cells invasion stimulated by TGF-beta. Similarly, lncRNA-ATB markedly enhanced TGF-beta-mediated invasion of glioma cells through activation P38 mitogen-activated protein kinase (P38/MAPK) pathway. Moreover, both the NF-kappa B selected inhibitor pyrrolidinedithiocarbamate ammonium and P38/MAPK specific inhibitor SB203580 partly reversed lncRNA-ATB induced glioma cells invasion mediated by TGF-beta. Collectively, this study revealed that lncRNA-ATB promotes TGF-beta-induced glioma cell invasion through NF-kappa B and P38/MAPK pathway and established a detailed framework for understanding the way how lncRNA-ATB performs its function in TGF-beta-mediated glioma invasion.

引用

页码：23302 / 23314

页数：13

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