New insight into isolation, identification techniques and medical applications of exosomes

被引:168
作者
Yang, Xiao-Xia [1 ]
Sun, Chao [1 ]
Wang, Lei [1 ]
Guo, Xiu-Li [1 ]
机构
[1] Shandong Univ, Sch Pharmaceut Sci, Dept Pharmacol, Drug Screening Unit Platform,Minist Educ,Key Lab, Jinan 250012, Shandong, Peoples R China
关键词
Exosomes; Isolation; Characterization; Drug/gene delivery vector; Cancer marker; CELL-DERIVED EXOSOMES; MESENCHYMAL STEM-CELL; EXTRACELLULAR VESICLES; TRANSFERRIN RECEPTOR; CANCER EXOSOMES; IN-VITRO; MEDIATED DELIVERY; STROMAL CELLS; MICRORNA; BRAIN;
D O I
10.1016/j.jconrel.2019.07.021
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Exosomes, which are nano-vesicles produced by most cell types, play an irreplaceable role in cell-cell communication. They are extracellular small vesicles that can delivery various cargos of DNA, RNAs, proteins, and lipids. Because exosomes have different secretory components under physiological conditions and pathological conditions, it has been extensively studied in the field of diseases as a therapeutic target, as a drug/gene delivery vector and as a novel cancer marker. Despite the great development in recent decades, there are still many obstacles to be overcome, for example, the separation method is not standardized with low yield and poor stability, which limit its medical application. This review mainly summarizes the main progresses of isolation and identification techniques, diversity function and medical application of exosomes in recent years.
引用
收藏
页码:119 / 129
页数:11
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