Function of untranslated regions in the mouse spermatogenesis-specific gene Tcp10 evaluated in transgenic mice

被引：6

作者：

Ewulonu, UK ^{[1
]}

Schimenti, JC ^{[1
]}

机构：

[1] JACKSON LAB, BAR HARBOR, ME 04609 USA

来源：

DNA AND CELL BIOLOGY | 1997年 / 16卷 / 05期

关键词：

D O I：

10.1089/dna.1997.16.645

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mouse Tcp10 genes are transcribed exclusively in male germ cells and display multiple 5' and 3' untranslated variations generated by alternative splicing and polyadenylation signal usage. To investigate the possible role of untranslated sequences in the regulation of these gems, chimeric expression constructs with or without endogenous 5' and 3' untranslated sequences were generated and used to make transgenic mice. Analysis of these animals showed that the untranslated sequences have no effect on the transcription or translation of an attached lacZ reporter gene, thereby implying these sequences are dispensible. However, the endogenous pattern of polyadenylation site usage,vas altered when Tcp10 3' untranslated sequences were linked to lacZ, indicating that internal coding sequence can influence recognition of polyadenylation signals in testis. The characteristics of alternative splicing and polyadenylation signal variability reflects a common theme of promiscuity in testicular gene expression.

引用

页码：645 / 651

页数：7

共 40 条

[1] IRON REGULATES FERRITIN MESSENGER-RNA TRANSLATION THROUGH A SEGMENT OF ITS 5' UNTRANSLATED REGION [J].