The complete nucleotide sequence and genomic organization of citrus leprosis associated virus, cytoplasmatic type (CiLV-C)

被引：50

作者：

Pascon, Renata C. ^{[1
]}

Kitajima, Joao Paulo ^{[1
]}

Breton, Michele C. ^{[1
]}

Assumpcao, Laura ^{[1
]}

Greggio, Christian ^{[1
]}

Zanca, Almir S. ^{[1
]}

Okura, Vagner Katsumi ^{[1
]}

Alegria, Marcos C. ^{[1
]}

Camargo, Maria E. ^{[1
]}

Silva, Giovana G. C. ^{[1
]}

Cardozo, Jussara C. ^{[1
]}

Vallim, Marcelo A. ^{[1
]}

Franco, Sulamita F. ^{[1
]}

Silva, Vitor H. ^{[1
]}

Jordao Junior, Hamilton ^{[1
]}

Oliveira, Fernanda ^{[1
]}

Giachetto, Poliana F. ^{[1
]}

Ferrari, Fernanda ^{[1
]}

Aguilar-Vildoso, Carlos I. ^{[1
]}

Franchiscini, Fabricio J. B. ^{[1
]}

Silva, Jose M. F. ^{[1
]}

Arruda, Paulo ^{[1
]}

Ferro, Jesus A. ^{[1
]}

Reinach, Fernando ^{[1
]}

Rasera da Silva, Ana Claudia ^{[1
]}

机构：

[1] Alellyx Appl Genom, BR-13069380 Campinas, SP, Brazil

来源：

VIRUS GENES | 2006年 / 32卷 / 03期

关键词：

citrus leprosis; citrus leprosis associated virus; citrus disease; Citrus sinensis; Tobamovirus; genome sequence;

D O I：

10.1007/s11262-005-6913-1

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The Citrus leprosis disease (CiL) is associated to a virus (CiLV) transmitted by Brevipalpus spp. mites (Acari: Tenuipalpidae). CiL is endemic in Brazil and its recently spreading to Central America represents a threat to citrus industry in the USA. Electron microscopy images show two forms of CiLV: a rare nuclear form, characterized by rod-shaped naked particle (CiLV-N) and a common cytoplasmic form (CiLV-C) associated with bacilliform-enveloped particle and cytoplasmic viroplasm. Due to this morphological feature, CiLV-C has been treated as Rhabdovirus-like. In this paper we present the complete nucleotide sequence and genomic organization of CiLV-C. It is a bipartite virus with sequence similarity to ssRNA positive plant virus. RNA1 encodes a putative replicase polyprotein and an ORF with no known function. RNA2 encodes 4 ORFs. pl5, p24 and p61 have no significant similarity to any known proteins and p32 encodes a protein with similarity to a viral movement protein. The CiLV-C sequences are associated with typical symptoms of CiL by RT-PCR. Phylogenetic analysis suggests that CiLV-C is probably a member of a new family of plant virus evolutionarily related to Tobamovirus.

引用

页码：289 / 298

页数：10

共 33 条

[1] BASIC LOCAL ALIGNMENT SEARCH TOOL [J].

ALTSCHUL, SF ;

GISH, W ;

MILLER, W ;

MYERS, EW ;

LIPMAN, DJ .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410

[2]

[Anonymous], PLANT DIS

[3] Otubains: a new family of cysteine proteases in the ubiquitin pathway [J].

Balakirev, MY ;

Tcherniuk, SO ;

Jaquinod, M ;

Chroboczek, J .

EMBO REPORTS, 2003, 4 (05) :517-522

[4]

BITANCOURT AA, 1937, O BIOL, V3, P37

[5]

BITANCOURT AA, 1955, ARQUIVOS I BIOL SAO, V2, P161

[6] Multiple sequence alignment with the Clustal series of programs [J].

Chenna, R ;

Sugawara, H ;

Koike, T ;

Lopez, R ;

Gibson, TJ ;

Higgins, DG ;

Thompson, JD .

NUCLEIC ACIDS RESEARCH, 2003, 31 (13) :3497-3500

[7]

Colariccio Addolorata, 1995, Fitopatologia Brasileira, V20, P208

[8] Base-calling of automated sequencer traces using phred.: II.: Error probabilities [J].

Ewing, B ;

Green, P .

GENOME RESEARCH, 1998, 8 (03) :186-194

[9]

Fawcett H., 1936, CITRUS DIS THEIR CON

[10] Evolution and origins of tobamoviruses [J].

Gibbs, A .

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES, 1999, 354 (1383) :593-602

← 1 2 3 4 →