MAXIMIZING THE PEAK PRODUCTION RATE IN OFF-LINE COMPREHENSIVE TWO-DIMENSIONAL LIQUID CHROMATOGRAPHY WITH MASS SPECTROMETRY DETECTION

被引：0

作者：

Eeltink, Sebastiaan ^{[1
]}

Dolman, Sebastiaan ^{[1
]}

Ursem, Mario ^{[1
]}

Swart, Remco ^{[1
]}

McLeod, Fraser ^{[1
]}

Schoenmakers, Peter J. ^{[2
]}

机构：

[1] Dionex Chem Corp, Amsterdam, Netherlands

[2] Univ Amsterdam, Polymer Anal Grp, Amsterdam, Netherlands

来源：

LC GC EUROPE | 2009年 / 22卷 / 08期

关键词：

CAPACITY; PERFORMANCE; SEPARATION; COLUMNS; SYSTEMS; SPEED; SIZE;

D O I：

暂无

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This article describes an optimization strategy to obtain the best possible performance in the shortest analysis time - called the peak production rate - for comprehensive off-line two-dimensional liquid chromatography. The demands on column technology (particle sized column length) and LC conditions (including sampling time, gradient time and slope, and column-equilibrium time) are discussed. The potential of off-line 2D-LC coupled to tandem mass spectrometry detection is demonstrated with the analysis of proteomics samples of various complexities and a trade-off is made between the 1D-LC and off-line 2D-LC performance.

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页码：404 / +

页数：7

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