Preparation and characterization of brush-like PEGMA-graft-PDA coating and its application for protein separation by CE

被引：17

作者：

Chen, Lijuan ^{[1
]}

Liu, Guangming ^{[2
]}

Liu, Songtao ^{[1
]}

Bai, Longchao ^{[1
]}

Wang, Yanmei ^{[1
]}

机构：

[1] Univ Sci & Technol China, Dept Polymer Sci & Engn, CAS Key Lab Soft Matter Chem, Hefei 230026, Peoples R China

[2] Univ Sci & Technol China, Dept Chem Phys, Hefei Natl Lab Phys Sci Microscale, Hefei 230026, Peoples R China

来源：

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION | 2014年 / 25卷 / 12期

基金：

中国国家自然科学基金;

关键词：

brush-like copolymer; polydopamine; protein resistance; protein separation; QUARTZ-CRYSTAL MICROBALANCE; CAPILLARY ELECTROCHROMATOGRAPHY; POLYMER BRUSHES; SURFACE MODIFICATION; POLYETHYLENE OXIDE; FACILE PREPARATION; STATIONARY-PHASE; CHAIN-LENGTH; POLYDOPAMINE; COPOLYMER;

D O I：

10.1080/09205063.2014.932267

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

In this paper, a novel brush-like copolymer consisting of poly(ethylene glycol) methyl ether methacrylate and 2-aminoethyl methacrylate (AEMA) named as poly(PEGMA(300)-co-AEMA) was synthesized by atom transfer radical polymerization (ATRP), and then, poly(PEGMA(300)-co-AEMA) copolymer was immobilized onto material surfaces through polydopamine (PDA)-anchored coating. The defined copolymer structure was characterized by nuclear magnetic resonance hydrogen spectroscopy (H-1 NMR) and gel permeation chromatography (GPC). The chemical component and surface morphology of the brush-like copolymer-graft-PDA coating were studied by using X-ray photoelectron spectroscopy (XPS) and scanning electron microscope (SEM), respectively. The hydrophilicity of the brush-like copolymer-graft-PDA coating was investigated by using static water contact angle. The protein-resistant property of the brush-like copolymer-graft-PDA coating was investigated by using quartz crystal microbalance with dissipation (QCM-D), and finally the coating was applied to capillary inner surface for protein separation by capillary electrophoresis (CE).

引用

页码：1306 / 1327

页数：22

共 53 条

[1] Quartz crystal microbalance-with dissipation monitoring (QCM-D) for real time measurements of blood coagulation density and immune complement activation on artificial surfaces [J].