Revisiting the structure of arabinogalactan from Lycium barbarum and the impact of its side chain on anti-ageing activity

被引：42

作者：

Huang, Wenqi ^{[1
]}

Zhao, Mengwei ^{[1
]}

Wang, Xinyi ^{[1
]}

Tian, Yang ^{[1
]}

Wang, Chengjian ^{[1
]}

Sun, Jing ^{[1
]}

Wang, Zhongfu ^{[1
]}

Gong, Guiping ^{[1
]}

Huang, Linjuan ^{[1
]}

机构：

[1] Northwest Univ, Coll Food Sci & Technol, Shaanxi Nat Carbohydrate Resource Engn Res Ctr, Xian 710069, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2022年 / 286卷

基金：

中国国家自然科学基金;

关键词：

Anti-ageing; Arabinogalactan; Lycium barbarum polysaccharides; Structure characterization; BIOLOGICAL-ACTIVITIES; POLYSACCHARIDES; IDENTIFICATION; EXPRESSION;

D O I：

10.1016/j.carbpol.2022.119282

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

In this study, we obtained all the homogeneous LBGP70 (arabinogalactan-protein complex), LBGP70-OL (sugar chain of LBGP70), and LBGP70-OL-I (backbone part of LBGP70-OL) fraction from crude Lycium barbarum polysaccharides (LBP) by stepwise precipitation. The structural features of LBGP70-OL were investigated by a series of analytical techniques and chemical methods, which was identified as a highly branched polysaccharide with an average of 9 branches per 10 sugar backbone units. The backbone was only -> 6)-beta-Galp-(1 -> residues, substituted at the C3 position. The side chains contained beta-Galp-(1 ->, alpha-Araf-(1 ->, alpha-Araf-(1 & RARR; 3)-beta-Araf-(1 -> 3)beta-Araf-(1 ->, alpha-Araf-(1 & RARR; 3)-beta-Gap-(1 -> 3)-beta-Galp-(1 ->, alpha-Araf-(1 & RARR; [5)-beta-Araf-(1]4 -> 3)-beta-Galp-(1 ->. LBGP70-OL had the highest anti-ageing activity, with its side chain and backbone exhibiting a synergistic effect. LBGP70-OL exerted the anti-ageing activity by attenuating SA-beta-Gal activity, preventing cell cycle arrest, increasing antioxidant enzyme activity, protecting the cell membranes from oxidative damage, and regulating the senescence related genes Sirt1 , NAMPT , and Prx1. These results lay the fundamental for further studies on the structure function relationships of LBP.

引用

页数：11

共 51 条

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