A new technique for implanting a fine-wire microelectrode for chronic recording of unit activity from freely-moving mice

被引：4

作者：

Oka, J ^{[1
]}

Imanishi, M ^{[1
]}

机构：

[1] Meijo Univ, Fac Pharmaceut Sci, Dept Chem Pharmacol, Tempaku Ku, Nagoya, Aichi 4688503, Japan

来源：

NEUROSCIENCE RESEARCH | 2000年 / 36卷 / 01期

关键词：

chronic recording; fine-wire; hippocampus; magnet; mouse; single unit; sleep;

D O I：

10.1016/S0168-0102(99)00106-6

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We report here a newly developed chronic implantation technique using an epoxy-coated fine-stainless steel wire (33 mu m in diameter) to record single unit activity from the brain of freely-moving mice with as little tissue injury as possible. Since the fine-wire electrode is not capable of staying straight by itself or of penetrating into the brain, a pair of permanent neodymium magnets placed on a micromanipulator as well as below the animal's head was used for stereotaxic implantation to keep the fine-wire straight and strong by the magnetic fields. With those implanted electrodes recording of single units from the hippocampal CAI of freely-moving mice was performed during sleep and wakefulness. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：93 / 96

页数：4