Maintenance of hepatocyte functions by Coculture with bone marrow stromal cells

被引：35

作者：

Isoda, K ^{[1
]}

Kojima, M ^{[1
]}

Takeda, M ^{[1
]}

Higashiyama, S ^{[1
]}

Kawase, M ^{[1
]}

Yagi, K ^{[1
]}

机构：

[1] Osaka Univ, Grad Sch Pharmaceut Sci, Suita, Osaka 5650871, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2004年 / 97卷 / 05期

关键词：

hepatocyte; bone marrow stromal cell; interleukin-6; albumin secretion; urea synthesis;

D O I：

10.1016/S1389-1723(04)70217-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Rat hepatocytes and bone marrow stromal cells (BMSCs) were cocultured with the aim of maintaining differentiated hepatocyte functions. After BMSCs were expanded to a confluent monolayer, freshly isolated hepatocytes were cultured with them, separated by a semipermeable membrane. The BMSCs significantly increased the urea synthesis and albumin secretion activities of the hepatocytes. Conditioned medium prepared from a BMSC monoculture had the same effect. Further study showed that interleukin-6 was involved in the maintenance of urea synthesis and another factor in the maintenance of albumin secretion.

引用

页码：343 / 346

页数：4

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