Spatial Localization of the Ebola Virus Glycoprotein Mucin-Like Domain Determined by Cryo-Electron Tomography

被引：44

作者：

Tran, Erin E. H. ^{[1
]}

Simmons, James A. ^{[2
]}

Bartesaghi, Alberto ^{[1
]}

Shoemaker, Charles J. ^{[2
]}

Nelson, Elizabeth ^{[2
]}

White, Judith M. ^{[2
]}

Subramaniam, Sriram ^{[1
]}

机构：

[1] NCI, Ctr Canc Res, NIH, Bethesda, MD 20892 USA

[2] Univ Virginia, Sch Med, Dept Cell Biol, Charlottesville, VA 22908 USA

来源：

JOURNAL OF VIROLOGY | 2014年 / 88卷 / 18期

关键词：

ANTIBODY; ENTRY; PROTECTION; PROTEINS; EPITOPES; BINDING;

D O I：

10.1128/JVI.00870-14

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Ebola virus glycoprotein mucin-like domain (MLD) is implicated in Ebola virus cell entry and immune evasion. Using cryo-electron tomography of Ebola virus-like particles, we determined a three-dimensional structure for the full-length glycoprotein in a near-native state and compared it to that of a glycoprotein lacking the MLD. Our results, which show that the MLD is located at the apex and the sides of each glycoprotein monomer, provide a structural template for analysis of MLD function.

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收藏

页码：10958 / 10962

页数：5

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