Molecular Characterization of Staphylococcus aureus Isolated from Meat and Their Antibiotic Resistance Profiles

被引:0
作者
Jaradat, Ziad W. [1 ]
Tarazi, Yaser H. [2 ]
Ababneh, Qotaibah O. [3 ]
机构
[1] Jordan Univ Sci & Technol, Fac Sci & Arts, Dept Biotechnol & Genet Engn, Irbid 22110, Jordan
[2] Jordan Univ Sci & Technol, Fac Vet Med, Dept Basic Vet Med Sci, Irbid 22110, Jordan
[3] Texas A&M Univ, Dept Biochem & Biophys, College Stn, TX 77843 USA
关键词
Antibiotics; Coagulase gene; Meat; RFLP; S; aureus; COAGULASE-NEGATIVE STAPHYLOCOCCI; PCR; MASTITIS; BOVINE; GENE; SUSCEPTIBILITY; IDENTIFICATION; PRODUCTS; TURKEY; BRAZIL;
D O I
暂无
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
The aims of this study were to characterize S. aureus isolates from different meat sources in Jordan and study their genetic relationship using PCR-RFLP in addition to their antibiotic resistance profiles. Thirty S. aureus isolates were identified and confirmed by PCR techniques. The isolates from goat and camel meats were sensitive to the majority of the tested antibiotics. Plasmid profiling revealed that 26 isolates contained at least one plasmid with no correlation between the number of plasmids and the resistance profiles. PCR-RFLP of the coagulase gene (coa) classified the isolates to several clusters upon digestion with Alu I or Cfo I restriction enzymes. This study concluded that the 30 S. aureus isolates were genetically diverse and comprised heterogeneous population with 7 genotypes at both 33.1 and 51.2 similarity levels. (C) 2013 PVJ. All rights reserved
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页码:58 / 62
页数:5
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