Deficient Fas ligand expression by synovial lymphocytes from patients with rheumatoid arthritis

被引:45
作者
Cantwell, MJ [1 ]
Hua, T [1 ]
Zvaifler, NJ [1 ]
Kipps, TJ [1 ]
机构
[1] UNIV CALIF SAN DIEGO,SCH MED,LA JOLLA,CA 92093
来源
ARTHRITIS AND RHEUMATISM | 1997年 / 40卷 / 09期
关键词
D O I
10.1002/art.1780400914
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective. To examine the expression and function of CD95 (Fas) and its ligand in rheumatoid arthritis (RA). Methods. We used flow cytometry and reverse transcriptase-polymerase chain reaction methods to assess lymphocyte expression of CD95 and its ligand. We also examined whether lymphocytes could undergo Fas-mediated apoptosis with anti-CD95 monoclonal antibody (MAb) or human Fas ligand-expressing fibroblasts, and if synovial fluid contained a soluble factor(s) that could inhibit such interactions. Finally, we determined whether anti-CD3 MAb could induce synovial T cells to express the Fas ligand in vitro. Results, Nearly all RA synovial fluid or synovial tissue lymphocytes expressed CD95 and could be induced to undergo apoptosis by CD95 crosslinking. We did not detect a soluble inhibitor in Ri synovial fluid that could account for the survival of CD95+ synovial cells in vivo, Instead, we detected little or no expression of Fas ligand by RA synovial lymphocytes. However, we could induce such cells to express Fas ligand with anti-CD3 MAb or phorbol ester and ionomycin in vitro. Conclusion. There is ineffective clearance of activated cells in the RA joint due to deficient expression of the Fas ligand.
引用
收藏
页码:1644 / 1652
页数:9
相关论文
共 42 条
  • [1] Die and let live: Eliminating dangerous lymphocytes
    Abbas, AK
    [J]. CELL, 1996, 84 (05) : 655 - 657
  • [2] THE AMERICAN-RHEUMATISM-ASSOCIATION 1987 REVISED CRITERIA FOR THE CLASSIFICATION OF RHEUMATOID-ARTHRITIS
    ARNETT, FC
    EDWORTHY, SM
    BLOCH, DA
    MCSHANE, DJ
    FRIES, JF
    COOPER, NS
    HEALEY, LA
    KAPLAN, SR
    LIANG, MH
    LUTHRA, HS
    MEDSGER, TA
    MITCHELL, DM
    NEUSTADT, DH
    PINALS, RS
    SCHALLER, JG
    SHARP, JT
    WILDER, RL
    HUNDER, GG
    [J]. ARTHRITIS AND RHEUMATISM, 1988, 31 (03): : 315 - 324
  • [3] Expression of fas antigen and Fas ligand in the rheumatoid synovial tissue
    Asahara, H
    Hasumuna, T
    Kobata, T
    Yagita, H
    Okumura, K
    Inoue, H
    Gay, S
    Sumida, T
    Nishioka, K
    [J]. CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY, 1996, 81 (01): : 27 - 34
  • [4] Baker SJ, 1996, ONCOGENE, V12, P1
  • [5] CELL-AUTONOMOUS FAS (CD95) FAS-LIGAND INTERACTION MEDIATES ACTIVATION-INDUCED APOPTOSIS IN T-CELL HYBRIDOMAS
    BRUNNER, T
    MOGIL, RJ
    LAFACE, D
    YOO, NJ
    MAHBOUBI, A
    ECHEVERRI, F
    MARTIN, SJ
    FORCE, WR
    LYNCH, DH
    WARE, CF
    GREEN, DR
    [J]. NATURE, 1995, 373 (6513) : 441 - 444
  • [6] PROTECTION FROM FAS-MEDIATED APOPTOSIS BY A SOLUBLE FORM OF THE FAS MOLECULE
    CHENG, JH
    ZHOU, T
    LIU, CD
    SHAPIRO, JP
    BRAUER, MJ
    KIEFER, MC
    BARR, PJ
    MOUNTZ, JD
    [J]. SCIENCE, 1994, 263 (5154) : 1759 - 1762
  • [7] HOW B-CELLS AND T-CELLS TALK TO EACH OTHER
    CLARK, EA
    LEDBETTER, JA
    [J]. NATURE, 1994, 367 (6462) : 425 - 428
  • [8] DANIEL PT, 1994, J IMMUNOL, V152, P5624
  • [9] Deleuran B W, 1996, Scand J Rheumatol Suppl, V104, P1
  • [10] AUTOCRINE T-CELL SUICIDE MEDIATED BY APO-1/(FAS/CD95)
    DHEIN, J
    WALCZAK, H
    BAUMLER, C
    DEBATIN, KM
    KRAMMER, PH
    [J]. NATURE, 1995, 373 (6513) : 438 - 441