Purification of recombinant cholera toxin polypeptide A2 and reconstitution with the cholera toxin B subunit.

被引：0

作者：

McCann, JA ^{[1
]}

Picking, WD ^{[1
]}

机构：

[1] ST LOUIS UNIV,DEPT BIOL,ST LOUIS,MO 63103

来源：

PROTEIN AND PEPTIDE LETTERS | 1997年 / 4卷 / 01期

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D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Polypeptide A2 of cholera toxin (CTA2) was cloned, expressed and purified as a fusion protein possessing a leader with six tandem histidine residues. Following affinity purification, recombinant CTA2 was labeled with a fluorescent probe at its single cysteine and reconstituted with the cholera toxin B subunit (CTB) pentamer. This novel CTB/CTA2 complex will be useful for monitoring the position of the site of attachment of the cytotoxic Al polypeptide of cholera toxin relative to sites on CTB or G(M1)-containing membranes.

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页码：39 / 46

页数：8

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