Purification Methodology for Viable and Infective Plasmodium vivax Gametocytes That Is Compatible with Transmission-Blocking Assays

被引：10

作者：

Vera, Omaira ^{[1
,2
]}

de Brito, Paula Brelas ^{[2
]}

Albrecht, Letusa ^{[1
,3
]}

Monick Martins-Campos, Keillen ^{[2
,4
]}

Pimenta, Paulo F. P. ^{[5
]}

Monteiro, Wuelton M. ^{[2
,4
]}

Lacerda, Marcus V. G. ^{[2
,6
]}

Lopes, Stefanie C. P. ^{[1
,2
,6
]}

Costa, Fabio T. M. ^{[1
]}

机构：

[1] Univ Estadual Campinas, UNICAMP, Dept Genet Evolucao & Bioagentes, Sao Paulo, Brazil

[2] Fundacao Med Trop Dr Heitor Vieira Dourado, Gerencia Malaria, Manaus, Amazonas, Brazil

[3] Fiocruz MS, Fundacao Oswaldo Cruz, Inst Carlos Chagas, Curitiba, Parana, Brazil

[4] Univ Estado Amazonas, Escola Super Ciencias Saude, Manaus, Amazonas, Brazil

[5] Fiocruz MS, Fundacao Oswaldo Cruz, Ctr Pesquisas Rene Rachou, Belo Horizonte, MG, Brazil

[6] Fiocruz MS, Fundacao Oswaldo Cruz, Inst Leonidas Maria Deane, Manaus, Amazonas, Brazil

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2015年 / 59卷 / 10期

基金：

巴西圣保罗研究基金会;

关键词：

MALARIA; BLOOD;

D O I：

10.1128/AAC.01136-15

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Significant progress toward the control of malaria has been achieved, especially regarding Plasmodium falciparum infections. However, the unique biology of Plasmodium vivax hampers current control strategies. The early appearance of P. vivax gametocytes in the peripheral blood and the impossibility of culturing this parasite are major drawbacks. Using blood samples from 40 P. vivax-infected patients, we describe here a methodology to purify viable gametocytes and further infect anophelines. This method opens new avenues to validate transmission-blocking strategies.

引用

页码：6638 / 6641

页数：4

共 12 条

[1] Epidemiology and Infectivity of Plasmodium falciparum and Plasmodium vivax Gametocytes in Relation to Malaria Control and Elimination [J].

Bousema, Teun ;

Drakeley, Chris .

CLINICAL MICROBIOLOGY REVIEWS, 2011, 24 (02) :377-+

[2] On the Cytoadhesion of Plasmodium vivax-Infected Erythrocytes [J].

Carvalho, Bruna O. ;

Lopes, Stefanie C. P. ;

Nogueira, Paulo A. ;

Orlandi, Patricia P. ;

Bargieri, Daniel Y. ;

Blanco, Yara C. ;

Mamoni, Ronei ;

Leite, Juliana A. ;

Rodrigues, Mauricio M. ;

Soares, Irene S. ;

Oliveira, Tatiane R. ;

Wunderlich, Gerhard ;

Lacerda, Marcus V. G. ;

del Portillo, Hernando A. ;

Araujo, Maria O. G. ;

Russell, Bruce ;

Suwanarusk, Rossarin ;

Snounou, Georges ;

Renia, Laurent ;

Costa, Fabio T. M. .

JOURNAL OF INFECTIOUS DISEASES, 2010, 202 (04) :638-647

[3] Emerging Plasmodium vivax resistance to chloroquine in South America: an overview [J].

Goncalves, Ligia Antunes ;

Cravo, Pedro ;

Ferreira, Marcelo Urbano .

MEMORIAS DO INSTITUTO OSWALDO CRUZ, 2014, 109 (05) :534-539

[4] Paucity of Plasmodium vivax Mature Schizonts in Peripheral Blood Is Associated With Their Increased Cytoadhesive Potential [J].

Lopes, Stefanie C. P. ;

Albrecht, Letusa ;

Carvalho, Bruna O. ;

Siqueira, Andre M. ;

Thomson-Luque, Richard ;

Nogueira, Paulo A. ;

Fernandez-Becerra, Carmen ;

del Portillo, Hernando A. ;

Russell, Bruce M. ;

Renia, Laurent ;

Lacerda, Marcus V. G. ;

Costa, Fabio T. M. .

JOURNAL OF INFECTIOUS DISEASES, 2014, 209 (09) :1403-1407

[5] Infection of Laboratory-Colonized Anopheles darlingi Mosquitoes by Plasmodium vivax [J].

Moreno, Marta ;

Tong, Carlos ;

Guzman, Mitchel ;

Chuquiyauri, Raul ;

Llanos-Cuentas, Alejandro ;

Rodriguez, Hugo ;

Gamboa, Dionicia ;

Meister, Stephan ;

Winzeler, Elizabeth A. ;

Maguina, Paula ;

Conn, Jan E. ;

Vinetz, Joseph M. .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2014, 90 (04) :612-616

[6] 1912-2012: a century of research on Plasmodium vivax in vitro culture [J].

Noulin, Florian ;

Borlon, Celine ;

Van Den Abbeele, Jan ;

D'Alessandro, Umberto ;

Erhart, Annette .

TRENDS IN PARASITOLOGY, 2013, 29 (06) :286-294

[7] Malaria in Brazil: an overview [J].

Oliveira-Ferreira, Joseli ;

Lacerda, Marcus V. G. ;

Brasil, Patricia ;

Ladislau, Jose L. B. ;

Tauil, Pedro L. ;

Daniel-Ribeiro, Claudio Tadeu .

MALARIA JOURNAL, 2010, 9

[8] Global extent of chloroquine-resistant Plasmodium vivax: a systematic review and meta-analysis [J].

Price, Ric N. ;

von Seidlein, Lorenz ;

Valecha, Neena ;

Nosten, Francois ;

Baird, J. Kevin ;

White, Nicholas J. .

LANCET INFECTIOUS DISEASES, 2014, 14 (10) :982-991

[9] Concentration and purification by magnetic separation of the erythrocytic stages of all human Plasmodium species [J].

Ribaut, Clotilde ;

Berry, Antoine ;

Chevalley, Severine ;

Reybier, Karine ;

Morlais, Isabelle ;

Parzy, Daniel ;

Nepveu, Francoise ;

Benoit-Vical, Francois ;

Valentin, Alexis .

MALARIA JOURNAL, 2008, 7 (1)

[10] Experimental Plasmodium vivax infection of key Anopheles species from the Brazilian Amazon [J].

Rios-Velasquez, Claudia M. ;

Martins-Campos, Keillen M. ;

Simoes, Rejane C. ;

Izzo, Thiago ;

dos Santos, Edineuza V. ;

Pessoa, Felipe A. C. ;

Lima, Jose B. P. ;

Monteiro, Wuelton M. ;

Secundino, Nagila F. C. ;

Lacerda, Marcus V. G. ;

Tadei, Wanderli P. ;

Pimenta, Paulo F. P. .

MALARIA JOURNAL, 2013, 12

← 1 2 →