Epimagnolin A inhibits IL-6 production by inhibiting p38/NF-κB and AP-1 signaling pathways in PMA-stimulated THP-1 cells

被引:21
作者
Chun, Hyun-Woo [1 ]
Kim, Soo-Jin [1 ]
Thu-Huyen Pham [1 ]
Bak, Yesol [1 ]
Oh, Jaewook [2 ]
Ryu, Hyung-Won [3 ]
Oh, Sei-Ryang [3 ]
Hong, Jin-Tae [4 ,5 ]
Yoon, Do-Young [1 ]
机构
[1] Konkuk Univ, Dept Biosci & Biotechnol, 120 Neungdong Ro, Seoul 05029, South Korea
[2] Konkuk Univ, Dept Stem Cell & Regenerat Biotechnol, Seoul, South Korea
[3] Korea Res Inst Biosci & Biotechnol, Nat Med Res Ctr, Cheongju, Chungcheongbuk, South Korea
[4] Chungbuk Natl Univ, Coll Pharm, Cheongju, Chungbuk, South Korea
[5] Chungbuk Natl Univ, Med Res Ctr, Cheongju, Chungbuk, South Korea
基金
新加坡国家研究基金会;
关键词
activator protein-1; anti-inflammatory; Epimagnolin A; NF-kappa B; p38; MPAK; THP-1; cells; NF-KAPPA-B; IN-VITRO; TRANSDUCTION PATHWAYS; RHEUMATOID-ARTHRITIS; MAGNOLIA-FARGESII; COX-2; EXPRESSION; FLOWER BUDS; KINASE; INFLAMMATION; RECEPTOR;
D O I
10.1002/tox.22746
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Epimagnolin A is a lignan obtained from the flower buds of Magnolia fargesii, which is traditionally used in Asian medicine for treating headache and nasal congestion. A herbal compound fargesin obtained from M. fargesii, has exerted anti-inflammatory effects in human monocytic THP-1 cells in the previous study. The anti-inflammatory effects of epimagnolin A, however, have been not elucidated yet. In this study, it was demonstrated that epimagnolin A reduced phorbol-12-myristate-13-acetate (PMA)-induced IL-6 promoter activity and IL-6 production in human monocytic THP-1 cells. Furthermore, it was investigated the modulating effects of epimagnolin A on mitogen-activated protein kinase, nuclear factor-kappa B (NF-kappa B), and activator protein 1 (AP-1) activities. Phosphorylation of p38 and nuclear translocation of p50 and c-Jun were down-regulated by epimagnolin A in the PMA-stimulated THP-1 cell. The results revealed that epimagnolin A attenuated the binding affinity of NF-kappa B and AP-1 transcription factors to IL-6 promoter and IL-6 production through p38/NF-kB and AP-1 signaling pathways in the PMA-stimulated THP-1 cells. These results suggest that epimagnolin A can be a useful drug for the treatment of inflammatory diseases.
引用
收藏
页码:796 / 803
页数:8
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