Growth of Cowdria ruminantium, the causative agent of heartwater, in a tick cell line

被引：31
作者：
Bell-Sakyi, L
Paxton, EA
Munderloh, UG
Sumption, KJ
机构：
[1] Univ Edinburgh, Ctr Trop Vet Med, Edinburgh, Midlothian, Scotland
[2] Univ Minnesota, Dept Entomol, St Paul, MN 55108 USA
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2000年 / 38卷 / 03期
关键词：
D O I：
10.1128/JCM.38.3.1238-1240.2000
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The tick-borne rickettsia Cowdria ruminantium has been propagated continuously for over 500 days in the Ixodes scapularis tick cell line IDES by using the Gardel isolate from bovine endothelial cells as an inoculum, Infection of the tick cells was confirmed by PCR, karyotyping, electron microscopy, and reinfection of bovine cells.
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页码：1238 / 1240
页数：3
相关论文
共 19 条
  • [1] ANDREASEN MP, 1974, ACTA PATH MICRO IM B, VB 82, P455
  • [2] BEZUIDENHOUT JD, 1985, ONDERSTEPOORT J VET, V52, P113
  • [3] Brown C. G. D., 1979, Practical tissue culture applications. (Proc. Conf. Internat. Lab. Res. Anim. Dis., Nairobi, Kenya, August 24-29 1978)., P223
  • [4] CAMUS E., 1996, Heartwater (cowdriosis): a review, V2nd
  • [5] Ewing S.A., 1995, P 76 C RES WORK AN D
  • [6] HART A, 1991, ONDERSTEPOORT J VET, V58, P187
  • [7] JONGEJAN F, 1991, ONDERSTEPOORT J VET, V58, P227
  • [8] KOCAN KM, 1987, ONDERSTEPOORT J VET, V54, P177
  • [9] IMMUNIZATION OF SHEEP AGAINST HEARTWATER WITH INACTIVATED COWDRIA-RUMINANTIUM
    MAHAN, SM
    ANDREW, HR
    TEBELE, N
    BURRIDGE, MJ
    BARBET, AF
    [J]. RESEARCH IN VETERINARY SCIENCE, 1995, 58 (01) : 46 - 49
  • [10] PROTECTION OF GOATS AGAINST HEARTWATER ACQUIRED BY IMMUNIZATION WITH INACTIVATED ELEMENTARY BODIES OF COWDRIA-RUMINANTIUM
    MARTINEZ, D
    MAILLARD, JC
    COISNE, S
    SHEIKBOUDOU, C
    BENSAID, A
    [J]. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 1994, 41 (1-2) : 153 - 163
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