Effect of Botulinum-A Toxin Injection into Bulbospongiosus Muscle on Ejaculation Latency in Male Rats

被引:26
作者
Serefoglu, Ege C. [1 ,2 ]
Hawley, Wayne R. [3 ]
Lasker, George F. [4 ]
Grissom, Elin M. [3 ]
Mandava, Sree H. [1 ]
Sikka, Suresh C. [1 ]
Dohanich, Gary P. [3 ]
Hellstrom, Wayne J. G. [1 ]
机构
[1] Tulane Univ, Sch Med, Dept Urol, New Orleans, LA 70112 USA
[2] Bagcilar Training & Res Hosp, Dept Urol, Istanbul, Turkey
[3] Tulane Univ, Dept Psychol, New Orleans, LA 70118 USA
[4] Tulane Univ, Sch Med, Dept Pharmacol, New Orleans, LA 70112 USA
关键词
Animal Models for Ejaculatory Behavior; Botulinum-A Toxin; Bulbospongiosus Muscle; Intromission; Premature Ejaculation; Sexual Behavior; LIFELONG PREMATURE EJACULATION; NEUROGENIC DETRUSOR OVERACTIVITY; SEROTONIN REUPTAKE INHIBITORS; EVIDENCE-BASED DEFINITION; SEXUAL DYSFUNCTION; INTERNATIONAL-SOCIETY; ERECTILE DYSFUNCTION; SEMEN PARAMETERS; DOUBLE-BLIND; DAPOXETINE;
D O I
10.1111/jsm.12553
中图分类号
R5 [内科学]; R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
1002 ; 100201 ;
摘要
Introduction. Premature ejaculation (PE) is the most common male sexual dysfunction. A variety of pharmacotherapeutic strategies have been employed to treat men suffering with lifelong PE. However, there are currently no pharmaceuticals approved by the U. S. Food and Drug Administration specifically designed for PE treatment. Aim. Given that the bulbospongiosus muscle is involved in the ejaculatory reflex in both humans and rodents and that local administration of botulinum-A can abolish muscle contractions, the current study examined the effect of injection of botulinum-A toxin into the bulbospongiosus muscle on the ejaculatory latency of male rats. Methods. After screening for normal sexual activity with sexually receptive female rats, 33 sexually experienced male Long-Evans rats (Harlan Laboratories, Indianapolis, IN, USA) underwent an additional four pretreatment sexual exposures over the course of the following week, during which all components of sexual behavior were video recorded by trained observers. On the day after their fourth experience, rats were anesthetized and received a single injection of either 0.5 unit (n = 11) or 1 unit (n = 11) of botulinum-A toxin or saline vehicle (n = 11). Botulinum-A toxin was dissolved in 0.1 mL of saline vehicle and injected bilaterally into the bulbospongiosus muscle by the percutaneous route. Beginning 2 days after treatment, sexual behaviors were reexamined over the course of the following week on four separate occasions. Main Outcome Measures. The latency to achieve ejaculation, and the frequencies and latencies of mounts and intromissions were video recorded by trained observers in a blinded fashion. Results. Relative to pretreatment measurements, bilateral injection of saline vehicle into the bulbospongiosus muscle did not affect ejaculation latencies. However, rats treated with either 0.5 or 1 unit of botulinum-A toxin exhibited significantly longer latencies to achieve ejaculation relative to pretreatment performance. Of note, botulinum-A toxin did not affect the ability to achieve mounts, intromissions, or ejaculation. Conclusions. These results demonstrate that botulinum-A toxin injection into the bulbospongiosus muscle is a safe and effective treatment that extends ejaculatory latency in rats without affecting the ability to engage in sexual activity or achieve ejaculation. Further studies are required to evaluate this therapeutic concept in PE patients.
引用
收藏
页码:1657 / 1663
页数:7
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