Comparison of sulfate-reducing and conventional Anammox upflow anaerobic sludge blanket reactors

被引：75
作者：
Rikmann, Ergo [1 ]
Zekker, Ivar [1 ]
Tomingas, Martin [1 ]
Vabamaee, Priit [1 ]
Kroon, Kristel [1 ]
Saluste, Alar [1 ]
Tenno, Taavo [1 ]
Menert, Anne [1 ]
Loorits, Liis [2 ]
Rubin, Sergio S. C. dC [3 ]
Tenno, Toomas [1 ]
机构：
[1] Univ Tartu, Inst Chem, EE-50411 Tartu, Estonia
[2] Tallinn Univ Technol, EE-19086 Tallinn, Estonia
[3] CNIB, Cala Cala, Cochabamba, Bolivia
来源：
JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2014年 / 118卷 / 04期
关键词：
Sulfate-reducing ammonium oxidation; Upflow anaerobic sludge blanket reactor; Humic matter; Anammox intermediates; Autotrophic NH4+ removal; SIMULTANEOUS REMOVAL; AMMONIUM OXIDATION; WASTE-WATER; START-UP; BACTERIA; SULFIDE; DIVERSITY; NITROGEN;
D O I：
10.1016/j.jbiosc.2014.03.012
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Autotrophic NH4+ removal has been extensively researched, but few studies have investigated alternative electron acceptors (for example, SO42-) in NH4+ oxidation. In this study, sulfate-reducing anaerobic ammonium oxidation (SRAO) and conventional Anammox were started up in upflow anaerobic sludge blanket reactors (UASBRs) at 36 (+/- 0.5)degrees C and 20 (+/- 0.5)degrees C respectively, using reject water as a source of NH4+. SO42- or NO2-, respectively, were applied as electron acceptors. It was assumed that higher temperature could promote the SRAO, partly compensating its thermodynamic disadvantage comparing with the conventional Anammox to achieve comparable total nitrogen (TN) removal rate. Average volumetric NH4+ - N removal rate in the sulfate-reducing UASBR1 was however 5-6 times less (0.03 kg-N/(m(3) day)) than in the UASBR2 performing conventional nitrite-dependent autotrophic nitrogen removal (0.17 kg-N/(m(3) day)). However, the stoichiometric ratio of NH4+ removal in UASBR1 was significantly higher than could be expected from the extent of SO42- reduction, possibly due to interactions between the N- and S-compounds and organic matter of the reject water. Injections of N2H4 and NH2OH accelerated the SRAO. Similar effect was observed in batch tests with anthraquinone-2,6-disulfonate (AQDS). For detection of key microorganisms PCR-DGGE was used. From both UASBRs, uncultured bacterium clone ATB-KS-1929 belonging to the order Verrucomicrobiales, Anammox bacteria (uncultured Planctomycete clone Pla_PO55-9) and aerobic ammonium-oxidizing bacteria (uncultured sludge bacterium clone ASB08 "Nitrosomonas") were detected. Nevertheless the SRAO process was shown to be less effective for the treatment of reject water, compared to the conventional Anammox. (c) 2014, The Society for Biotechnology, Japan. All rights reserved.
引用
收藏
页码：426 / 433
页数：8
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